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Apparatus for determination of a component in a sample

  • US 5,114,673 A
  • Filed: 09/12/1989
  • Issued: 05/19/1992
  • Est. Priority Date: 01/21/1988
  • Status: Expired due to Term
First Claim
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1. Apparatus for determining an analyte in a fluid sample, comprising:

  • (a) a first zone containing one component selected from the group consisting of (i) labeled analyte, (ii) labeled analyte analogue, and (iii) labeled receptor which specifically binds to said analyte, wherein said component dissolves in said fluid sample, and wherein if said component is labeled receptor, binds with said analyte to form a complex of analyte and labeled receptor,(b) a second zone which is in fluid contact with said first zone and which receives fluid sample after passing through said first zone, wherein said second zone contains a solid phase bound receptor which binds to the component previously in said first zone, with the proviso that said solid phase bound receptor does not bind to complexes of analyte and labeled receptor,(c) a third zone separated from said second zone by a means capable of being rendered fluid permeable, and containing a reactive compound which reacts with the label of said labeled analyte, labeled analyte analogue or labeled receptor when contacted thereto to form a detectable moiety, wherein said means (i) prevents passage of said reactive component into said second zone until completion of binding of said solid phase bound receptor to the component released from said first zone and (ii) permits passage of the component into said second zone thereafter wherein said third zone is positioned relative to said second zone to permit movement of said reactive component into said second zone when said means are rendered fluid permeable, and(d) a fourth zone in fluid contact with said second zone which removes excess fluid flowing from said second zone.

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