Optical sensor for determining at least one parameter in a liquid or gaseous sample

• US 5,114,676 A
• Filed: 07/28/1989
• Issued: 05/19/1992
• Est. Priority Date: 08/04/1988
• Status: Expired due to Term

• Current Assignee: AVL AG
• Original Assignee: AVL AG
• Inventors: Weiss, Leonie, Wolfbeis, Otto S., Leiner, Marco J.
• Primary Examiner(s): Hill, Jr., Robert J.
• Assistant Examiner(s): Chan, William
• Application Number:
  US07/386,151
• Time in Patent Office: 1,026 Days
• Field of Search: 204/403, 422/82.06, 422/82.07, 422/58, 422/61, 435/288, 435/817, 435/291, 128/634, 350/96.29, 350/96.35, 350/96.34
• US Class Current: 422/82.06
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 31/223 for investigating presence of specific gases or aerosols G01N31/221, G01N31/222 take precedence; actuation of fire alarm by presence of smoke or gases G08B17/10
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

In order to improve the response behavior of an optical sensor for determining at least one parameter in a liquid or gaseous sample, the sensor including a substrate which is provided with carrier particles and a fluorescent indicator immobilized thereon, the substrate is configured as a polymer film transparent to both excitation and emission radiation, and the individual carrier particles carrying the fluorescent indicator in immobilized form are bonded with only part of their surface to a thin layer of a thermoplastic material adhering to the polymer film and assume thermosetting properties after the carrier particles have been pressed in, whereas the other part of the surface of the carrier particles extend into an optically transparent hydrogel layer which covers the thermoplastic layer and in which the carrier particles are anchored mechanically. Such sensors may easily be attached to the end of an optical waveguide and may be made in any size by a simple punching operation.

Claims

1. An optical thin-film sensor for determining at least one parameter in a liquid or gaseous sample, comprising:

a polymer support film which is transparent to both excitation and emission radiation;

a thin, transparent layer of a thermoplastic material adhered to said polymer film;

an optically transparent hydrogel layer which covers said thermoplastic layer; and

carrier particles having a diameter and a thickness with a fluorescent indicator immobilized thereon, a first part of said carrier particles being bonded to said thermoplastic material, having thermosetting properties after said carrier particles have been pressed in, and a second part of said carrier particles extending into said optically transparent hydrogel layer, said hydrogel layer being mechanically anchored by said carrier particles.

2. An optical sensor according to claim 1, wherein the diameter of the individual carrier particles is larger than the thickness of said layer adhering to said polymer film.

3. An optical sensor according to claim 1, including a layer of pigmented hydrogel covering said hydrogel layer.

4. An optical sensor according to claim 1, including a pH-sensitive fluorescent indicator immobilized on said carrier particles.

5. An optical sensor according to claim 4, including a CO₂ -permeable, ion-impermeable membrane attached on said optical sensor for pCO₂ measurement.

6. An optical sensor according to claim 1, including an enzyme fromt the group consisting of hydrolases, oxidases and dehydrogenases immobilized in said hydrogel layer.

7. An optical sensor according to claim 1, wherein said carrier particles are configured as individual fibers having a surface, a small first part of the surface of said fibers is fixed in said layer adhering to said polymer film, while a larger second part of the surface of said fibers is held in said hydrogel layer.

8. An optical sensor according to claim 7, wherein a fibrous layer of microcrystalline cellulose fibers is provided, said cellulose fibers carrying a fluorescent indicator immobilized by covalent bonding.

9. An optical sensor according to claim 8, wherein said individual cellulose fibers have a diameter of 5-10 μ

m and a length of 50-200 μ

m.

10. An optical sensor according to claim 9, wherein said individual cellulose fibers have a diameter of about 8 μ

m and a length of about 80 μ

m.

11. An optical sensor according to claim 7, wherein said optically transparent hydrogel layer is covered by a layer of dyed hydrogel.

12. An optical sensor according to claim 7, including an enzyme from the group consisting of hydrolases, oxidases and dehydrogenases immobilized on said carrier particles.

13. An optical sensor according to claim 1, wherein said polymer film has a thickness of 20-500 μ

m, while said layer adhering to said polymer film has a thickness of 5-10 μ

m, and said individual hydrogel layer has a thickness of 5-30 μ

m.