Superfusion apparatus
First Claim
1. A perfusion device for the analysis of samples comprising:
- (a) a housing having means for controlling the temperature of said housing;
(b) an opening in said housing;
(c) a sample reaction chamber having a first end and a second end and a configuration for fitting inside said opening in said housing;
(d) a sample reaction chamber plug having a first end and a second end and a passage connecting said first end with said second end and sealing means at said first end for engaging and providing a fluid tight seal between said sample reaction chamber plug and said first end of said sample reaction chamber; and
(e) electrical stimulation means disposed in said first end of said sample reaction chamber plug.
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Accused Products
Abstract
A superfusion apparatus for the controlled reaction of a sample within a test chamber through controlled exposure to a series of reagents in a set test sequence is provided for electrical or chemical stimulation to determine if a receptor binding compound is an agonist or antagonist. The superfusion apparatus provides a mass production but meticulously controlled sample environment for controlling temperature, quantity and arrangement of reagents and wash fluids to provide a high reproducability of results which may be compuer assisted. The device has a fully automated mode or a manually operated mode. The fully automated mode allows for the storage and execution of a number of different tests to be performed on the samples due to the provision of desired sequences of test parameters.
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Citations
20 Claims
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1. A perfusion device for the analysis of samples comprising:
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(a) a housing having means for controlling the temperature of said housing; (b) an opening in said housing; (c) a sample reaction chamber having a first end and a second end and a configuration for fitting inside said opening in said housing; (d) a sample reaction chamber plug having a first end and a second end and a passage connecting said first end with said second end and sealing means at said first end for engaging and providing a fluid tight seal between said sample reaction chamber plug and said first end of said sample reaction chamber; and (e) electrical stimulation means disposed in said first end of said sample reaction chamber plug. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A device for the analysis of biological samples comprising:
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(a) a housing having means for controlling the temperature of said housing; (b) a plurality of openings in said housing; (c) a plurality of substantially hollow reaction chambers having ends and an outside configuration suitable for fitting inside said plurality of openings in said housing; (d) a plurality of reaction chamber plugs each having a first end and a second end and a passage connecting said first end with said second end and means for sealing said first end to an end of one of said plurality of substantially hollow reaction chambers; and (e) pump means for pumping fluids through said passage in said plurality of reaction chamber plugs and said plurality of substantially hollow reaction chambers. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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17. A superfusion device for treating biological samples comprising:
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(a) a housing having means for controlling the temperature of said housing; (b) a plurality of openings, in said housing; (c) a plurality of substantially hollow reaction chambers having ends and an outside configuration suitable for fitting inside said plurality of openings in said housing; (d) a plurality of reaction chamber plugs each having a first end and a second end and a passage connecting said first end with said second end and means for electrical stimulation disposed in said first end; and (e) a peristaltic pump for pumping fluids through said passage in said plurality of reaction chamber plugs and said plurality of substantially hollow reaction chambers. - View Dependent Claims (18, 19, 20)
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Specification