Superfusion apparatus

• US 5,114,681 A
• Filed: 03/09/1990
• Issued: 05/19/1992
• Est. Priority Date: 03/09/1990
• Status: Expired due to Fees

• Current Assignee: Biomedical Research and Development Laboratories Incorporated
• Original Assignee: Biomedical Research and Development Laboratories Incorporated
• Inventors: Evans, Robert, Kroener, Calvin, Cobb, Wayne, Bertoncini, Joseph
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Trembley, T. A.
• Application Number:
  US07/491,717
• Time in Patent Office: 802 Days
• Field of Search: 422/110, 422/111, 422/186, 422/186.04, 422/186.1, 422/186.2, 422/50, 422/63, 422/65, 422/68.1, 422/78, 422/102, 422/104, 250/328, 435/287, 435/290, 435/313, 435/316, 435/173
• US Class Current: 422/111
• CPC Class Codes: G01N 2001/305 Fixative compositions
• CPC Class Codes: G01N 2035/00435 Refrigerated reagent storage
• CPC Class Codes: G01N 2035/102 Preventing or detecting loss of fluid by dripping
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 35/00584 Control arrangements for automatic analysers
• CPC Class Codes: G01N 35/00603 Reinspection of samples
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums

Abstract

A superfusion apparatus for the controlled reaction of a sample within a test chamber through controlled exposure to a series of reagents in a set test sequence is provided for electrical or chemical stimulation to determine if a receptor binding compound is an agonist or antagonist. The superfusion apparatus provides a mass production but meticulously controlled sample environment for controlling temperature, quantity and arrangement of reagents and wash fluids to provide a high reproducability of results which may be compuer assisted. The device has a fully automated mode or a manually operated mode. The fully automated mode allows for the storage and execution of a number of different tests to be performed on the samples due to the provision of desired sequences of test parameters.

Claims

1. A perfusion device for the analysis of samples comprising:

(a) a housing having means for controlling the temperature of said housing;

(b) an opening in said housing;

(c) a sample reaction chamber having a first end and a second end and a configuration for fitting inside said opening in said housing;

(d) a sample reaction chamber plug having a first end and a second end and a passage connecting said first end with said second end and sealing means at said first end for engaging and providing a fluid tight seal between said sample reaction chamber plug and said first end of said sample reaction chamber; and

(e) electrical stimulation means disposed in said first end of said sample reaction chamber plug.

2. The perfusion device of claim 1 wherein said electrical stimulation means is a platinum electrode.

3. The perfusion device of claim 1 wherein said first end of said sample reaction chamber includes means for removably engaging said sample reaction chamber from said opening in said housing.

4. The perfusion device of claim 3 wherein said means for removably engaging said sample reaction chamber from said opening is two notches in said first end of said sample reaction chamber.

5. The perfusion device of claim 1 further comprising a second sample reaction chamber plug having a first end and a second end and a passage connecting said first end with said second end and sealing means at said first end for providing a fluid tight seal between said second sample reaction chamber plug and said sample reaction chamber.

6. The perfusion device of claim 5 further comprising electrical stimulation means disposed in said first end of said second sample reaction chamber plug.

7. The perfusion device of claim 5 wherein said first end of said sample reaction chamber includes means for selectively removing said sample reaction chamber on said first or on said second sample reaction chamber plug.

8. The perfusion device of claim 7 wherein said means for selectively removing said sample reaction chamber is two notches in said first end of said sample reaction chamber.

9. The perfusion device of claim 5 wherein said passage connecting said first end and said second end of said first and said second sample reaction chamber plug is connected through tubing to a peristaltic pump.

10. A device for the analysis of biological samples comprising:

(a) a housing having means for controlling the temperature of said housing;

(b) a plurality of openings in said housing;

(c) a plurality of substantially hollow reaction chambers having ends and an outside configuration suitable for fitting inside said plurality of openings in said housing;

(d) a plurality of reaction chamber plugs each having a first end and a second end and a passage connecting said first end with said second end and means for sealing said first end to an end of one of said plurality of substantially hollow reaction chambers; and

(e) pump means for pumping fluids through said passage in said plurality of reaction chamber plugs and said plurality of substantially hollow reaction chambers.

11. The device of claim 10 wherein one of the ends of said plurality of substantially hollow reaction chambers includes means for removably engaging said sample reaction chamber from said plurality of openings in said housing.

12. The device of claim 11 further comprising electrical stimulation means disposed in said first end of said plurality of reaction chamber plugs.

13. The device of claim 11 further comprising a plurality of tubing having an intake end and a discharge end connected to said pump means and moveable trays for supporting test tubes at said intake end of said discharge end of said tubing.

14. The device of claim 13 further comprising means for introducing gasses to said intake end of said tubing.

15. The device of claim 13 further comprising computer control means for moving said moveable trays.

16. The device of claim 13 further comprising means for controlling the temperature of said test tubes at said intake end and said discharge end of said tubing.

17. A superfusion device for treating biological samples comprising:

(a) a housing having means for controlling the temperature of said housing;

(b) a plurality of openings, in said housing;

(c) a plurality of substantially hollow reaction chambers having ends and an outside configuration suitable for fitting inside said plurality of openings in said housing;

(d) a plurality of reaction chamber plugs each having a first end and a second end and a passage connecting said first end with said second end and means for electrical stimulation disposed in said first end; and

(e) a peristaltic pump for pumping fluids through said passage in said plurality of reaction chamber plugs and said plurality of substantially hollow reaction chambers.

18. The superfusion device of claim 17 further comprising a plurality of tubing having an intake end and a discharge end connected to said peristaltic pump and moveable trays disposed at said intake end and said discharge end of said tubing.

19. The superfusion device of claim 18 further comprising computer control means for controlling said means for controlling the temperature of said housing and for controlling the movement of said moveable trays.

20. The superfusion device of claim 18 wherein said peristaltic pump is capable of forward and reverse pumping.