Process for DNA sequencing using oligonucleotide bank
First Claim
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1. Process for DNA sequencing by the multi-primer method which comprises;
- (a) selecting in the DNA to be sequenced a single-stranded primer-attachment site of known sequence;
(b) hybridizing immediately adjacent to each other at the primer-attachment site two oligonucleotides selected from the group consisting of hexameric, heptameric, octameric and nonameric oligonucleotides obtained from a bank comprising all possible 46, 47, 48 an d49 different oligonucleotides;
(c) hybridizing simultaneously with step (b) or after step (b) a further hexameric, heptameric, octameric or nonameric oligonucleotide immediately adjacent to one of the other two oligonucleotides at the primer-attachment site,(d) ligating the oligonucleotides to form a primer, and(e) heat treating to remove non-ligated oligonucleotides.
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Abstract
A process for DNA sequencing by the multi-primer method involving the use of oligonucleotide banks of hexamers, heptamers, octomers, or nonamers for the creation of the primer through the ligation of two or more oligomers following hybridization onto the primer site.
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3 Claims
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1. Process for DNA sequencing by the multi-primer method which comprises;
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(a) selecting in the DNA to be sequenced a single-stranded primer-attachment site of known sequence; (b) hybridizing immediately adjacent to each other at the primer-attachment site two oligonucleotides selected from the group consisting of hexameric, heptameric, octameric and nonameric oligonucleotides obtained from a bank comprising all possible 46, 47, 48 an d49 different oligonucleotides; (c) hybridizing simultaneously with step (b) or after step (b) a further hexameric, heptameric, octameric or nonameric oligonucleotide immediately adjacent to one of the other two oligonucleotides at the primer-attachment site, (d) ligating the oligonucleotides to form a primer, and (e) heat treating to remove non-ligated oligonucleotides. - View Dependent Claims (2, 3)
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Specification