Recombinant DNA, transformant containing said DNA, and process for preparing heat-stable glucose dehydrogenase by use of said transformant
First Claim
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1. An isolated DNA fragment encoding a thermostable glucose dehydrogenase, said thermostable glucose dehydrogenase having the following amino acid sequence:
- ##STR5## wherein A represents Ser or Ala, X represents Asp or Glu, Y represents Ala or Ser, and B represents Leu or Met; and
wherein Z1, Z2, and Z3, each represent a thermostability-conferring amino acid, provided that Z1, Z2, and Z3 taken together cannot be Glu, Gln, and Tyr, respectively.
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Abstract
A recombinant DNA replicable and expressible in Escherichia coli is disclosed which encodes glucose dehydrogenase originating from Bacillus megaterium. A DNA sequence encoding the glucose dehydrogenase is incorporated into an E. coli vector and then used to transform an E. coli host. Also disclosed are transformants and recombinant DNA incorporating thermostable glucose dehydrogenase, wherein at least one amino acid in glucose dehydrogenase at positions 96, 252, and 253 is replaced by a thermostability-conferring amino acid.
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9 Claims
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1. An isolated DNA fragment encoding a thermostable glucose dehydrogenase, said thermostable glucose dehydrogenase having the following amino acid sequence:
- ##STR5## wherein A represents Ser or Ala, X represents Asp or Glu, Y represents Ala or Ser, and B represents Leu or Met; and
wherein Z1, Z2, and Z3, each represent a thermostability-conferring amino acid, provided that Z1, Z2, and Z3 taken together cannot be Glu, Gln, and Tyr, respectively. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- ##STR5## wherein A represents Ser or Ala, X represents Asp or Glu, Y represents Ala or Ser, and B represents Leu or Met; and
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