Fiber optic sensors, apparatus, and detection methods using fluid erodible controlled release polymers for delivery of reagent formulations

• US 5,114,864 A
• Filed: 09/19/1989
• Issued: 05/19/1992
• Est. Priority Date: 06/25/1986
• Status: Expired due to Term

• Current Assignee: Trustees of Tufts College
• Original Assignee: Trustees of Tufts College
• Inventors: Walt, David R.
• Primary Examiner(s): Saunders, David
• Assistant Examiner(s): Chin, Christopher L.
• Application Number:
  US07/409,462
• Time in Patent Office: 973 Days
• Field of Search: 436/528, 436/166, 436/172, 436/800, 436/805, 422/82.07, 422/82.08, 422/82.05, 422/901, 424/426, 350/96.1, 350/96.2, 385/12, 385/13
• US Class Current: 436/528
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 2021/7793 Sensor comprising plural indicators
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

An improved fiber optic sensor, sensing apparatus, and methods for making optical determinations are provided. The fiber optic sensor employs a fiber optic strand to convey light energy and at least one polymer matrix comprising a fluid erodible, continuous release polymer and a releasable reagent formulation able to react with a molecule or analyte of interest. The optic sensors and sensor construction has been demonstrated to be accurate, precise, and of long duration.

Claims

1. A fiber optic sensor for performing optical determinations of a plurality of test fluids, said sensor comprising;

a housing with a securing means to hold a fiber optic strand, said housing comprising a reservoir chamber of fixed configuration and interior spatial volume comprised of at least one side wall and having at least one open portal accessing said interior spatial volume;

at least one polymeric matrix comprising a fluid contact positioned within said interior spatial volume of said reservoir chamber, said polymeric matrix comprising a fluid erodible, continuous release polymer and a sustained release reagent formulation above to react with a molecule of interest in each of the test fluids;

a fiber optic strand positioned proximal to said erodible polymeric matrix within said reservoir chamber, said fiber optic strand being able to convey light energy into and out of said interior spatial volume of said reservoir chamber; and

a conduit member of fixed dimensions and shape in fluid flow communication with said portal of said reservoir chamber, said conduit member being comprised of at least one side wall, at least one aperture perforating said wall, and a perforated internal volume in flow communication with said interior spatial volume of said reservoir chamber.

2. The fiber optic sensor as recited in claim 1 wherein said reagent formulation is selected from the group consisting of indicator compounds and dyes.

3. The fiber optic sensor as recited in claim 1 wherein said reagent formulation is selected from the group consisting of labelled immunological and immunochemical compositions.

4. The fiber optic sensor as recited in claim 1 wherein said reagent formulation is selected from the group consisting of enzyme system components and compositions.

5. The fiber optic sensor as recited in claim 1 wherein said continuous release polymer is a polyanhydride.

6. The fiber optic sensor as recited in claim 1 wherein said continuous release polymer is a vinyl polymer.

7. The fiber optic sensor as recited in claim 1 wherein said continuous release polymer is a polyacrylamide.

8. A fiber optic apparatus for performing optical determinations of a plurality of test fluids, said apparatus comprising:

a fiber optical sensor comprised of;

(i) a housing with a securing means to hold a fiber optic strand, said housing comprising a reservoir chamber of fixed configuration and interior spatial volume,

(ii) at least one polymeric matrix erodible by repetitious fluid contact positioned within said interior spatial volume of said reservoir chamber, said polymeric matrix comprising a fluid erodible continuous release polymer and a sustained release reagent formulation able to react with a molecule of interest in each of the test fluids,

(iii) a fiber optic strand positioned proximal to said erodible polymeric matrix within said reservoir chamber, said fiber optic strand being able to convey light energy into and out of said interior spatial volume of said reservoir chamber, and

(iv) a conduit member for allowing each of the test fluids into and out of said interior spatial volume of said reservoir chamber wherein said conduit member comprises at least one side wall, at least one aperture perforating said wall, and a perforated internal volume in flow communication with said interior spatial volume of said reservoir chamber;

a source of light energy;

means for conveying light energy from said source to said fiber optic sensor;

means for detecting light energy; and

means for conveying light energy from said fiber optic sensor to said detecting means.

9. The fiber optic sensing apparatus as recited in claim 8 further comprising a computer control and data acquisition system.

10. The fiber optic sensing apparatus as recited in claim 8 wherein said light energy is in the ultraviolet light range.

11. The fiber optic sensing apparatus as recited in claim 8 wherein said light energy is in the visible light range.

12. A method for making optical determinations of a plurality of test fluids, said method comprising the steps of:

obtaining a fiber optic sensor comprised of;

a housing with a securing means to hold a fiber optic strand, said housing comprising a reservoir chamber of fixed configuration and interior spatial volume,

(ii) at least one polymeric matrix erodible by repetitious fluid contact positioned within said interior spatial volume of said reservoir chamber, said polymeric matrix comprising a fluid erodible continuous release polymer and a sustained release reagent formulation able to react with a molecule of interest in each of the test fluids,

(iii) a fiber optic strand positioned proximal to said erodible polymeric matrix within said reservoir chamber, said fiber optic strand being able to convey light energy into and out of said interior spatial volume of said reservoir chamber, and

(iv) a conduit member for allowing each of the test fluids into and out of said interior spatial volume of said reservoir chamber wherein said conduit member comprises at least one side wall, at least one aperture perforating said wall, and a perforated internal volume in flow communication with said interior spatial volume of said reservoir chamber;

introducing each of the test fluids into said interior spatial volume of said reservoir chamber via said conduit member such that said polymeric matrix partially erodes and continuously releases said reagent formulation for mixture and reaction with said molecule of interest within each of said introduced test fluids;

conveying light energy to said fiber optic sensor such that said light energy interacts with each of said reaction mixtures comprised of released reagent formulation and said molecule of interest within said reservoir chamber; and

detecting the light energy conveyed by said fiber optic sensor after light interaction with each of said reaction mixtures comprised of released reagent formulation and said molecule of interest.