Nonenzymatic glucose test

• US 5,116,763 A
• Filed: 10/13/1988
• Issued: 05/26/1992
• Est. Priority Date: 11/19/1984
• Status: Expired due to Fees

• Current Assignee: Miles, Inc. (Bayer AG)
• Original Assignee: Miles, Inc. (Bayer AG)
• Inventors: Wu, Wen H., Ismail, Ibrahim A., Greene, Carmine
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): McMahon, Timothy M.
• Application Number:
  US07/256,559
• Time in Patent Office: 1,321 Days
• Field of Search: 422/56, 422/57, 436/95, 436/163, 435/14, 435/805, 427/2, 427/395
• US Class Current: 436/95
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

The invention provides a nonenzymatic method useful for the semiquantitative determination of glucose, a test composition and test device. Glucose concentration in an aqueous test sample can be determined by preparing a test solution by contacting an aqueous test sample and a dihydroxide component, at an initial pH above 6.5, capable of forming a complex with glucose which complex formation releases a proton, and determining the final pH of the test solution. The invention also provides a self-indicating device format which allows the determination of glucose concentration by merely counting the number of pads which have changed color. No comparison to a color chart is required. The glucose test of the present invention is free of the interferences found in enzymatic glucose tests.

Claims

1. A method for determining glucose concentration in a sample, said method comprising the steps of:

mixing a liquid sample with a test solution having an initial pH greater than 6.5 and consisting essentially of a borate buffer and pH indicator capable of detecting pH in the pH range of 6.5 to 12;

detecting the pH of the resulting mixture; and

correlating the detected pH to a glucose concentration for the liquid sample.

2. The method of claim 1 wherein the pH indicator is a dual indicator comprising bromothymol blue and cresol red.