Effective ophthalmic irrigation solution

• US 5,116,868 A
• Filed: 05/03/1989
• Issued: 05/26/1992
• Est. Priority Date: 05/03/1989
• Status: Expired due to Fees

• Current Assignee: Johns Hopkins University
• Original Assignee: Johns Hopkins University
• Inventors: Chen, Chung-Ho, Chen, Sumi C.
• Primary Examiner(s): Waddell, Frederick E.
• Assistant Examiner(s): Fay, Zohreh A.
• Application Number:
  US07/346,700
• Time in Patent Office: 1,119 Days
• Field of Search: 514/546, 514/912
• US Class Current: 514/546
• CPC Class Codes: A01N 1/021 Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
• CPC Class Codes: A61K 9/0048 Eye, e.g. artificial tears

Abstract

A composition comprising an aqueous phosphate-buffered balanced salt solution including glucose, an antioxidant, and ketone bodies and/or precursors thereof is disclosed. The disclosed composition is particularly useful as an ophthalmic irrigation solution which may be used in ocular surgery, although other uses, for example, ophthalmic topical application and surgeries in general, are also contemplated.

Claims

1. A method of applying an ophthalmic irrigation composition to ocular tissues to sustain tissue cell viability and capability to perform physiological functions in an amount sufficient to irrigate the tissues, said composition comprising an aqueous phosphate-buffered balanced salt solution including glucose, an antioxidant, and at least one of ketone bodies and precursors thereof in amounts sufficient to effectively meet requirements of ocular tissues for efficient physiological and biochemical functioning.

2. The method of claim 1, wherein the antioxidant is a member of the group consisting of citrate, phenylalanine, vitamin E and ascorbate.

3. The method of claim 2, wherein the antioxidant is citrate.

4. The method of claim 3, wherein the concentration of citrate is in the range of 8-12 mM.

5. The method of claim 1, wherein the ketone bodies are 62 -hydroxybutyrate and acetoacetate ions.

6. The method of claim 5, wherein the β

-hydroxybutyrate ions are selected from the group consisting of D-isomers of β

-hydroxybutyrate, and D- and L- racemic mixture of β

-hydroxybutyrate and a D- and L-isomer mixture of β

-hydroxybutyrate.

7. The method of claim 6, wherein the β

-hydroxybutyrate ions are the D-isomers of β

-hydroxybutyrate.

8. The method of claim 5, wherein the concentration of β

-hydroxybutyrate is in the range of 5-10 mM.

9. The method of claim 1, wherein the ketone body precursors are short-chain fatty acids selected from the group consisting of acetic acid and butyric acid, and ketogenic amino acids selected from the group consisting of leucine, lysine, phenylalanine, tyrosine and tryptophan.

10. The method of claim 9, wherein the concentration of the ketone body precursors is in the range of 0.1-10 mM.

11. The method of claim 9, wherein the ophthalmic irrigation solution comprises one ketogenic amino acid having a concentration in the range of 0.1-5.0 mM.

12. The method of claim 9, wherein the ophthalmic irrigation solution comprises one ketogenic amino acid having a concentration in the range of 1-2 mM.

13. The method of claim 9, wherein the ketogenic amino acids have a combined total concentration of in the range of 7.5-12.5 mM.

14. The method of claim 9, wherein the short-chain fatty acids are acetic acid at a concentration in the range of 15-25 mM, or butyric acis at a concentration in the range of 5-15 mM.

15. The method of claim 14, wherein the acetic acid is at a concentration of 20 mM.

16. The method of claim 14, wherein the butyric acid is at a concentration of 10 mM.

17. The method of claim 1, wherein the ophthalmic irrigation composition is applied to the ocular tissues in ocular surgery in an amount sufficient to replace at least one of aqueous humor and vitreous humor with the irrigation solution.

18. The method of claim 1 wherein the pH of the ophthalmic irrigation composition is adjusted to 7.3 to 7.5.