Material for affinity chromatography which is a sulfated polysaccharide bonded to an insoluble polymer

• US 5,116,962 A
• Filed: 07/16/1990
• Issued: 05/26/1992
• Est. Priority Date: 05/25/1985
• Status: Expired due to Term

• Current Assignee: CSL Behring GmbH (CSL Limited)
• Original Assignee: Behringwerke Aktiengesellschaft (Sanofi )
• Inventors: Paques, Eric-Paul, Stuber, Werner
• Primary Examiner(s): Brown, Johnnie R.
• Assistant Examiner(s): Peselev, Elli
• Application Number:
  US07/554,075
• Time in Patent Office: 680 Days
• Field of Search: 536/18.7, 536/51, 536/21, 424/81, 424/18, 424/83, 424/78, 525/54.2
• US Class Current: 525/54.2
• CPC Class Codes: B01J 20/262 obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G
• CPC Class Codes: B01J 20/264 derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers
• CPC Class Codes: B01J 20/265 modified or post-treated polymers polymer carriers or substrates subjected to further impregnating or coating B01J20/3208
• CPC Class Codes: B01J 20/286 Phases chemically bonded to a substrate, e.g. to silica or to polymers
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3217 Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3248 the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: C07K 14/8128 Antithrombin III
• CPC Class Codes: C08B 37/0075 Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof

Abstract

A process for the preparation of a material for affinity chromatography is described, in which a sulfated polysaccharide is bonded to a carrier material which has amino groups.

For this purpose, a sulfated polysaccharide is treated with an oxidizing agent which oxidizes glycols to aldehydes, with cleavage of the carbon chain, and this modified sulfated polysaccharide is allowed to react with a carrier which has amino groups.

It is possible using the described affinity material to adsorb proteins which bind to sulfated polysaccharides from solutions of these proteins and, where appropriate, then to desorb them, for example antithrombin III from blood plasma.

Claims

1. A sulfated polysaccharide bonded to an insoluble polymer prepared by a process which comprises treating a sulfated polysaccharide selected from the group consisting of heparin and its sodium salt with an alkali metal periodate which oxidizes glycols to aldehydes, thereby cleaving the carbon chain, and reacting the modified sulfated polysaccharide with an insoluble polymer containing amino groups selected from the group consisting of dextran resins, agarose resins and copolymers of methacrylic acid derivatives, pentaerythritol, polyethylene glycol and divinylbenzene.