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Restriction-fragment DNA probes and probe clusters

  • US 5,118,604 A
  • Filed: 06/15/1990
  • Issued: 06/02/1992
  • Est. Priority Date: 06/15/1990
  • Status: Expired due to Fees
First Claim
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1. A probe comprising:

  • a first DNA segment (1) derived from the upstream end region of a linear DNA fragment obtained by digestion of genomic DNA to completion with a restriction endonuclease which cuts at sites that occur infrequently, said DNA fragment having upstream and downstream end regions which are spaced from one another by between about 100 kilobases to 2,000 kilobases, and (2) which is effective to bind by homologous base pairing to said upstream end region in genomic DNA; and

    ,a second DNA segment (1) derived from the downstream end region of said DNA fragment and connected adjacent its downstream end, as defined by the upstream-to-downstream orientation in the DNA fragment, to the upstream end of the first segment, and (2) which is effective to bind by homologous base pairing to said downstream end region in genomic DNA;

    where the probe represents a subset of restriction fragments of said DNA, and the probe is of a size which can be cloned.

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