Process and device for the laser desorption of an analyte molecular ions, especially of biomolecules
First Claim
1. Process for the laser desorption of analyte molecular ions of biomolecules comprising the steps of locating a specimen in a range from 10,000 to several hundred thousand Dalton, providing a matrix for said analyte which absorbs laser light, and irradiating said specimen with laser light with a wavelength of about 300 nm or greater, said matrix being able to absorb said laser light.
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Abstract
The invention relates to a process for the laser desorption of analyte molecular ions, especially of biomolecules, from a specimen. The object of the invention is to improve a process of this type in such a way that intact desorption of biomolecules, preferably of large molecular mass, is also possible with a laser. This is achieved by using laser light of a wavelength of about 300 nm or greater than 300 nm and using a matrix which is able to absorb in the selected wavelength range.
280 Citations
34 Claims
- 1. Process for the laser desorption of analyte molecular ions of biomolecules comprising the steps of locating a specimen in a range from 10,000 to several hundred thousand Dalton, providing a matrix for said analyte which absorbs laser light, and irradiating said specimen with laser light with a wavelength of about 300 nm or greater, said matrix being able to absorb said laser light.
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27. Device for the laser desorption of analyte molecular ions, of biomolecules, with a molecular mass in the range from 10,000 to several hundred thousand Dalton, from a specimen which contains the analyte comprising a laser for producing light of a wavelength of about 300 nm or greater, said biomolecules not absorbing said laser light;
- and a matrix for said analyte which absorbs said laser light.
- View Dependent Claims (28, 29, 30, 31, 32, 33, 34)
Specification
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- Resources
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Current AssigneeSequenom Incorporated (Laboratory Corporation Of America Holdings)
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Original AssigneeFinnigan Mat GmbH
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InventorsKaras, Michael, Hillenkamp, Franz, Giessmann, Ulrich
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Primary Examiner(s)Anderson, Bruce C.
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Application NumberUS07/570,283Time in Patent Office651 DaysField of Search250/282, 250/288, 250/423 PUS Class Current250/282CPC Class CodesG01N 1/22 in the gaseous state specia...G01N 2001/045 Laser ablation; Microwave v...G01N 27/44704 Details; AccessoriesG01N 27/44717 Arrangements for investigat...G01N 30/72 Mass spectrometers mass spe...G01N 30/74 Optical detectors measureme...G01N 30/95 Detectors specially adapted...H01J 49/164 Laser desorption/ionisation...
