Biosensor and a process for preparation thereof
First Claim
1. A biosensor for determining a substrate concentration in a sample solution comprising a base plate having an electrode system and a reaction layer having formed thereon a space including said reaction layer, said space being provided with an introducing port for introducing said sample solution into said space and a discharge port for discharging the gas in said space by inflow of said sample solution, said electrode system being equipped with at least an electrode for measurement and a counter electrode, at least an enzyme being carried on said reaction layer, a change in concentration of a substance in the reaction between said enzyme and said sample solution being detected with said electrode system to determine a substrate concentration in said sample solution.
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Accused Products
Abstract
A biosensor of the invention comprises an insulating base board (1) having formed thereon, in sequence, leads (2, 3, 3'"'"'), an electrode system mainly made of carbon (4, 5, 5'"'"'), an insulating layer (6) and a reaction layer (14) composed of an enzyme and an electron acceptor, and being provided thereon with a space (8) defined by a spacer (7) and a cover 9). When a biological sample solution is brought into contact with the inlet (10) of the biosensor having the above-described structure, the sample solution is introduced into its inside, while the air within the space (8) is rapidly discharged through the outlet (11) and, at the same time, the space (8) is filled with the sample solution up to the neighborhood of the outlet. Thus, measurement can be conducted inexpensively at a high speed with a high accuracy through simple procedures without residual bubbles.
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Citations
18 Claims
- 1. A biosensor for determining a substrate concentration in a sample solution comprising a base plate having an electrode system and a reaction layer having formed thereon a space including said reaction layer, said space being provided with an introducing port for introducing said sample solution into said space and a discharge port for discharging the gas in said space by inflow of said sample solution, said electrode system being equipped with at least an electrode for measurement and a counter electrode, at least an enzyme being carried on said reaction layer, a change in concentration of a substance in the reaction between said enzyme and said sample solution being detected with said electrode system to determine a substrate concentration in said sample solution.
- 15. A process for preparing a biosensor which comprises forming an electrode system comprising at least an electrode for measurement and a counter electrode on an insulating base plate, coating a hydrophilic high molecular substance aqueous solution and an oxidoreductase aqueous solution on said electrode system and then drying to form an enzyme layer, spreading a mixture of an electron acceptor and an organic solvent onto said enzyme layer, removing said organic solvent to form an electron acceptor layer and then integrating together with a cover.
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16. A process for preparing a biosensor which comprises forming an electrode system comprising at least an electrode for measurement and a counter electrode on an insulating base plate, coating a hydrophilic high molecular substance aqueous solution and an oxidoreductase aqueous solution on said electrode system and then drying to form an enzyme layer, next spreading a solution of a hydrophilic high molecular substance in an organic solvent onto said enzyme layer to form a hydrophilic high molecular substance layer, further spreading a dispersion of an electron acceptor in an organic solvent onto said hydrophilic high molecular substance layer to form an electron acceptor layer, and then integrating together with a cover.
Specification