Apparatus and method for optically analyzing biological fluids

• US 5,122,284 A
• Filed: 04/01/1991
• Issued: 06/16/1992
• Est. Priority Date: 06/04/1990
• Status: Expired due to Term

• Current Assignee: Abaxis Incorporated (Zoetis Incorporated)
• Original Assignee: Abaxis Incorporated (Zoetis Incorporated)
• Inventors: Ostoich, Vladimir, Braynin, Boris, Burd, Tammy L.
• Primary Examiner(s): KIM, SUN U
• Application Number:
  US07/678,824
• Time in Patent Office: 442 Days
• Field of Search: 210/94, 210/95, 210/198.1, 210/380.1, 210/407, 210/512.1, 210/514, 210/515, 210/532.1, 210/745, 210/782, 210/787, 210/789, 422/64, 422/72, 422/101, 422/102, 436/45, 436/63, 436/177, 436/180, 494/16, 494/17, 494/27, 494/29, 494/37, 494/43, 494/10, 356/246, 356/427
• US Class Current: 210/782
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: G01N 2035/00237 Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A centrifugal rotor comprising a plurality of peripheral cuvettes and a collection chamber spaced radially inward from the cuvettes is disclosed. The rotor includes a means for introducing fluid into the collection chamber and plurality of generally radial inlet channels connecting each cuvette to the chamber. Each inlet channel has a discrete flow path for fluid to enter the cuvette and another discrete flow path for gas to exit the cuvette as the cuvette is filled. Positioned radially inward from each cuvette is a reflective surface capable of deflecting a light beam from a generally vertical direction to a generally horizontal direction or vice versa. As the rotor is spun, fluid enters the cuvettes from collection chamber through the inlet channels, which also allow gas in the cuvettes to escape, thus avoiding the creation of bubbles in the cuvette after the cuvettes are filled. The contents are then optically analyzed by passing a light beam horizontally through the cuvette.

Claims

1. A centrifugal rotor comprising a plurality of peripheral cuvettes, a collection chamber spaced radially inward from the cuvettes, a means for introducing a fluid into the chamber, and a plurality of generally radially inlet channels connecting each cuvette to the chamber, wherein each inlet channel comprises means for defining two contiguous flow paths, each flow path being separately defined and distinct, whereby the fluid enters the cuvette through a first flow path and gas exits the cuvette through a second flow path.

2. The rotor of claim 1, wherein the means for defining two contiguous flow paths comprises a first region having a smooth surface texture and a second region having a rough surface texture.

3. The rotor of claim 1, wherein the means for defining two contiguous flow paths comprises a first region having a greater depth than a second region.

4. The rotor of claim 1, wherein the means for defining two contiguous flow paths comprises a first region having a hydrophilic surface and a second region having a hydrophobic surface.

5. The rotor of claim 1, wherein the first flow path is on a side of the inlet channel in the direction of rotation of the rotor and the second flow path is on a side of the inlet channel away from the direction of the rotation of the rotor.

6. The rotor of claim 1, further comprising a separation chamber, having a cell trap, connected to the collection chamber.

7. The rotor of claim 6, wherein the collection chamber is disposed radially outward from the separation chamber.

8. The rotor of claim 1, wherein the fluid is blood plasma or diluted blood plasma.

9. The rotor of claim 1, wherein the rotor is formed from clear plastic.

10. The rotor of claim 1, wherein each cuvette contains reagents necessary for analysis of the fluid.

11. A centrifugal rotor comprising a plurality of peripheral cuvettes, a collection chamber spaced radially inward from the cuvettes and disposed below a separation chamber having a cell trap, an axial drainage port between the collection chamber and the separation chamber for introducing a biological fluid into the collection chamber, and a plurality of generally radial inlet channels connecting each cuvette to the collection chamber, wherein each inlet channel has a first discrete flow path for flow of the fluid into the cuvette as the rotor is spun and a second discrete flow path for flow of gas out of the cuvette as the rotor is spun.

12. A method for filling a peripheral cuvette in a centrifugal rotor with a fluid, the method comprising:

introducing the fluid into a collection chamber disposed radially inward of the cuvette;

spinning the rotor to effect the radially outward flow of the fluid through an inlet channel into the cuvette, wherein the inlet channel comprises means for defining two contiguous flow paths, each flow path being separately defined and distinct, whereby the fluid enters the cuvette through a first flow path and gas exits the cuvette through a second flow path.

13. A method of claim 12, further comprising separating the fluid from cellular material associated with the fluid before the step of introducing the fluid into the collection chamber.

14. A centrifugal rotor comprising a vertical axis, a plurality of peripheral cuvettes, and a plurality of reflective surfaces positioned radially inward from the cuvettes, wherein each reflective surface is oriented with respect to the vertical axis such that an incident light beam oriented generally parallel to the vertical axis is deflected to be oriented generally perpendicular to the vertical axis and an incident light beam oriented generally perpendicular to the vertical axis is deflected to be orientated generally parallel to the vertical axis.

15. The rotor of claim 14, wherein the reflective surface is oriented at about a 45 degree angle to the vertical axis of the rotor.

16. The rotor of claim 14, wherein the reflective surface is a mirror.

17. The rotor of claim 14, wherein at least a portion of the rotor comprises clear plastic.

18. The rotor of claim 17, wherein the clear plastic is acrylic.

19. The rotor of claim 14, wherein each cuvette contains reagents necessary for a biochemical analysis of the fluid.

21. A rotor of claim 14, further comprising a collection chamber spaced radially inward from the cuvettes, a means for introducing the fluid into the chamber, and a plurality of generally radial inlet channels connecting each cuvette to the chamber, wherein each inlet channel comprises means for defining two contiguous flow paths, each flow path being separately defined and distinct, whereby the fluid enters the cuvette through a first flow path and gas exits the cuvette through a second flow path.

20. The rotor of clam 19, wherein the biochemical analysis produces an optical effect when exposed to the light beam.

22. A method of optically analyzing a biological fluid in a centrifugal rotor having a vertical axis and a plurality of peripheral cuvettes, the method comprising:

(a) placing the fluid in a collection chamber in the rotor;

(b) spinning the rotor such that the fluid enters the cuvettes;

(c) detecting a light beam after it has been reflected by a reflective surface oriented at about a 45 degree angle to the vertical axis.

23. The method of claim 22, wherein the light beam is oriented generally parallel to the vertical axis and is reflected to be oriented generally perpendicular to the vertical axis.

24. The method of claim 22, wherein the light beam is oriented generally perpendicular to the vertical axis and is reflected to be oriented generally parallel to the vertical axis.

25. A centrifugal rotor comprising a vertical axis, a plurality of peripheral cuvettes, a plurality of reflective surfaces oriented at about a 45 degree angle to the vertical axis and positioned radially inward from the cuvettes, a collection chamber spaced radially inward from the reflective surfaces, and a plurality of generally radially inlet channels connecting each cuvette to the chamber, wherein each inlet channel comprises means for defining two contiguous flow paths, each flow path being separately defined and distinct, whereby a fluid enters the cuvette through a first flow path and gas exits the cuvette through a second flow path.