Polyanhydride bioerodible controlled release implants for administration of stabilized growth hormone

• US 5,122,367 A
• Filed: 03/31/1989
• Issued: 06/16/1992
• Est. Priority Date: 03/31/1989
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology, Nova Pharmaceutical Corporation
• Inventors: Chasin, Mark, Turek, Tom, Langer, Robert S., Ron, Eyal
• Primary Examiner(s): Page, Thurman K.
• Assistant Examiner(s): Kulkosky, Peter F.
• Application Number:
  US07/332,554
• Time in Patent Office: 1,173 Days
• Field of Search: 424/78, 424/80, 424/426, 424/81
• US Class Current: 424/426
• CPC Class Codes: A61K 38/27 Growth hormone [GH], i.e. somatotropin
• CPC Class Codes: A61K 47/26 Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• CPC Class Codes: A61K 9/2018 Sugars, or sugar alcohols, e.g. lactose, mannitol; Derivatives thereof, e.g. polysorbates
• CPC Class Codes: A61K 9/2031 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyethylene oxide, poloxamers

Abstract

A controlled release device for the administration of biologically active growth hormone proteins or peptide fragments, and method of preparation thereof, wherein biologically active growth hormone is stabilized and release rate modulated by incorporation of a stabilizing compound. The controlled release devices are prepared by mixing a stabilizer, such as sucrose, with a biologically active growth hormone, such as bovine somatotropic hormone, in solution, lyophilizing, then incorporating the dried powder into a surface erodible, biocompatible polymeric matrix, such as a poly(anhydride) or poly(orthoester) matrix.

Claims

1. A controlled release system for a protein comprising:

a protein having physical, chemical and biological activity equivalent to that of somatotrophin;

a simple polysaccharide; and

a biocompatible, surface erodible, hydrophobic polymeric matrix selected from the group consisting of polyanhydrides, poly(orthoesters), copolymers and combinations thereof, wherein the polysaccharide and protein are incorporated within and released from the polymeric matrix, the ratio of polysaccharide to protein in the matrix is greater than 1;

1, and the rate of release of the protein in combination with the polysaccharide is more linear than the rate or release of the protein in the absence of the polysaccharide.

2. The controlled release system of claim 1 wherein the protein is elected from the group consisting of polypeptides, peptide fragments and natural, recombinant and synthetic analogs thereof of somatotrophin, having somatotrophin activity.

3. The controlled release system of claim 1 wherein the polysaccharide is selected from the group consisting of sucrose, glucose, and fructose.

4. The controlled release system of claim 1 wherein the polysaccharide is sucrose, the growth hormone like protein is biologically active growth hormone, and the polymeric matrix is formed from poly(anhydrides).

10. The delivery system of claim 1 wherein the polysaccharide to protein ratio is between approximately 2:

1 and 8;

1.

5. A method for making a delivery system for a protein comprising providing a protein having physical, chemical and biological activity equivalent to that of somatotrophin in combination with a simple polysaccharide and incorporating the combination within a biocompatible, surface erodible, hydrophobic polymeric matrix selected from the group consisting of polyanhydrides, poly(orthoesters), copolymers and combinations thereof, wherein the polysaccharide and protein are incorporated within and released from the polymeric matrix, the ratio of polysaccharide to protein is greater than 1:

1 and the rate of release of the protein in combination with the polysaccharide is more linear than the rate of release of the protein in the absence of the polysaccharide.

6. The method of claim 5 further comprising lyophilizing a solution containing the protein and polysaccharide before incorporation into the polymer matrix.

7. The method of claim 5 wherein the polysaccharide is selected from the group consisting of sucrose, glucose, and fructose.

8. The method of claim 5 wherein the protein is selected from the group consisting of polypeptides, peptide fragments and natural, recombinant and synthetic analogs thereof of somatotrophin, having somatotrophin activity.

9. The method of claim 5 wherein the polysaccharide is sucrose, the growth hormone-like protein is biologically active growth hormone, and the polymeric matrix is formed from poly(anhydrides).

11. The method of claim 5 wherein the polysaccharide to protein ratio is between approximately 2:

1 and 8;

1.