Dry strip element for the electrochemical detection of theophylline
First Claim
1. A dry strip element for use in an electrochemical assay method for detecting theophyline, said element comprising a working electrode and a reference electrode and separately comprising at the working electrode an alkaline phosphatase and an electroinactive phosphate ester which is a substrate for the alkaline phosphatase and from which an electroactive compound can be released by catalytic activity of the alkaline phosphatase.
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Abstract
A dry strip element for use in an electrochemical assay for determining theophylline in a sample, the test element carrying a working electrode and a reference electrode and separately carrying at the working electrode an alkaline phosphatase and an electroinactive phosphate ester which is a substrate for the alkaline phosphatase and from which an electroactive compound can be released by catalytic activity of the alkaline phosphatase.
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Citations
9 Claims
- 1. A dry strip element for use in an electrochemical assay method for detecting theophyline, said element comprising a working electrode and a reference electrode and separately comprising at the working electrode an alkaline phosphatase and an electroinactive phosphate ester which is a substrate for the alkaline phosphatase and from which an electroactive compound can be released by catalytic activity of the alkaline phosphatase.
- 5. A dry strip element according to claim 11, which incorporates a buffer to buffer the pH at 9 to 10 during the assay said buffer being positioned between the region of sample application and the alkaline phosphatase.
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7. A dry strip element according to claim 11, in which the alkaline phosphatase is positioned so as to protect said substrate from the sample allowing said alkaline phosphatase to be contacted by the sample before the phosphate ester.
Specification