Detection of diamines in biological fluids

• US 5,124,254 A
• Filed: 10/02/1990
• Issued: 06/23/1992
• Est. Priority Date: 02/08/1988
• Status: Expired due to Fees

• Current Assignee: Cardiff University, Welsh Medical School Enterprises Limited
• Original Assignee: Cardiff University, Welsh Medical School Enterprises Limited
• Inventors: Davis, Robert H., Winterburn, Peter J., Hewlins, Michael J. E., O'Dowd, Thomas C.
• Primary Examiner(s): Nucker, Christine
• Assistant Examiner(s): PRESTON, DAVID
• Application Number:
  US07/555,412
• Time in Patent Office: 630 Days
• Field of Search: 435/29, 435/34, 435/25, 435/28
• US Class Current: 435/28
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/26 involving oxidoreductase

Abstract

A fast-acting and portable diagnostic kit for detecting diamines in biological fluids is in the form of a diamine oxidase and a chromogenic system to detect the presence of hydrogen peroxide resulting from oxidation of such diamines. The kit may especially be used to detect cadaverine or putrescine or a mixture thereof, for example in a vaginal secretion, in order to detect Gardnerella-related bacterial vaginosis.

Claims

1. A method for the detection of putrescine and cadaverine in vaginal secretions to diagnose vaginal infection comprising the steps of:

adding a sample of vaginal fluid to a mixture comprising;

a diamine oxidase having a high specificity for a diamine selected from the group consisting of putrescine and cadaverine, and which reacts with putrescine and cadaverine to form hydrogen peroxide; and

a chromogenic system to detect the presence of elevated levels of hydrogen peroxide resulting from the reaction between said diamine oxidase and putrescine and cadaverine.

2. A method according to claim 1, wherein the sample is obtained by a swab.

3. A method according to claim 1, wherein the diamine oxidase is derived from pea seedlings.

4. A method according to claim 1, wherein the chromogenic system is composed of peroxidase, 4-aminoantipyrine, and a phenolic compound.

5. A method according to claim 4, wherein the phenolic compound is 3,5-dichloro-2-hydroxbenzenesulphonic acid.

6. A method according to claim 1, wherein the vaginal infection is Gardnerella-related bacterial vaginosis.

7. A diagnostic kit useful for the direct testing of vaginal fluid to detect the presence of elevated levels of putrescine and cadaverine for diagnosis of vaginal infection, consisting essentially of the following elements in at least one container:

a premeasured amount of diamine oxidase which is highly specific for a diamine selected from the group consisting of putrescine and cadaverine, and which reacts with putrescine and cadaverine to form hydrogen peroxide;

a premeasured amount of a chromogenic system responsive to hydrogen peroxide;

said diamine oxidase and said chromogenic system being disposed in said at least one container container.

8. The kit of claim 7 wherein said diamine oxidase and said chromogenic system are in freeze dried form.

9. The kit of claim 7 wherein said diamine oxidase is derived from pea seedlings.

10. The kit of claim 7 wherein said chromogenic system comprises peroxidase, 4-aminoantipyrine and a phenolic compound.

11. The kit of claim 10 wherein the phenolic compound is 3,5-dichloro-2-hydroxybenzenesulphonic acid.

12. A diagnostic device useful for the direct testing of vaginal fluid to detect the presence of elevated levels of putrescine and cadaverine for diagnosis of vaginal infection, consisting essentially of a paper or other surface layer absorbed with a test composition consisting essentially of a premeasured amount of diamine oxidase which is highly specific for a diamine selected from the group consisting of putrescine and cadaverine, and which reacts with putrescine and cadaverine to form hydrogen peroxide, and a premeasured amount of a chromogenic system responsive to hydrogen peroxide.

13. The device of claim 12 wherein said diamine oxidase is derived from pea seedlings.

14. The device of claim 12 wherein said chromogenic system comprises peroxidase, 4-aminoantipyrine and a phenolic compound.

15. The device of claim 14 wherein said phenolic compound is 3,5-dichloro-2-hydroxybenzenesulphonic acid.