Process for the determination of a specifically bindable substance
First Claim
1. Method for determination of a specifically bindable substance comprising:
- incubating a liquid sample with at least three receptors R1, R2 and R3, wherein(a) R1 specifically binds with R2, R3 and said specifically bindable substance and is an antibody or an Fab2 fragment;
(b) R2 comprises a conjugate of (i) a substance S which is identical to the specifically bindable substance to be determined and (ii) a first partner P1 of a binding pair,(c) R3 comprises a conjugate of S and a label, to form complexes containing R1, R2 and R3,(d) adding a second partner of a specific binding pair P2 to said liquid sample together with a solid phase, wherein P2 binds to said solid phase and to P1 to immobilize said complexes containing R1, R2 and R3, and,determining R3 bound to solid phase or in liquid phase as a determination of said specifically bindable substance.
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Accused Products
Abstract
The present invention provides a process for the determination of a specifically bindable substance by incubation of a sample solution with at least three receptors R1, R2 and R3, of which R1 is specifically bindable with R2 and R3, as well as with the substance to be determined, R2 brings about the binding to a solid phase and R3 carries a labelling, separation of bound labelling from unbound labelling and measurement of the labelling in one of the two phases, wherein, as receptor R1, a receptor is used which has at least two binding positions which bind specifically with an epitope of the substance to be determined, as R2 a conjugate of a partner P1 of a specifically binding pair and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, the partner P1 thereby either being bound to a solid phase or being immobilized, and as R3 a complex is used which contains at least the substance S and a labelling.
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Citations
18 Claims
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1. Method for determination of a specifically bindable substance comprising:
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incubating a liquid sample with at least three receptors R1, R2 and R3, wherein (a) R1 specifically binds with R2, R3 and said specifically bindable substance and is an antibody or an Fab2 fragment; (b) R2 comprises a conjugate of (i) a substance S which is identical to the specifically bindable substance to be determined and (ii) a first partner P1 of a binding pair, (c) R3 comprises a conjugate of S and a label, to form complexes containing R1, R2 and R3, (d) adding a second partner of a specific binding pair P2 to said liquid sample together with a solid phase, wherein P2 binds to said solid phase and to P1 to immobilize said complexes containing R1, R2 and R3, and, determining R3 bound to solid phase or in liquid phase as a determination of said specifically bindable substance. - View Dependent Claims (5, 7, 9, 11, 13, 15, 17)
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2. Method for determination of a specifically bindable substance, comprising:
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incubating a liquid sample with at least three receptors R1, R2 and R3, and with a solid phase bound partner P2 of a binding pair, wherein; a) R1 specifically binds with R2, R3 and said specifically bindable substance and is an antibody or an Fab2 fragment; (b) R2 comprises a conjugate of (i) a substance S which is identical to the specifically bindable substance to be determined and (ii) a first partner P1 of a binding pair; (c) R3 comprises a conjugate of S and a label, (d) P2 binds to P1, to form immobilized complexes containing R1, R2 and R3, and; determining R3 bound to solid phase or in liquid phase as a determination of said specifically bindable substance. - View Dependent Claims (3, 4, 6, 8, 10, 12, 14, 16, 18)
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Specification