Probe for measuring oxygen concentration

• US 5,128,102 A
• Filed: 01/03/1989
• Issued: 07/07/1992
• Est. Priority Date: 07/03/1986
• Status: Expired due to Fees

• Current Assignee: Rikagaku Kenkyusho, Terumo Kabushiki Kaisha
• Original Assignee: Rikagaku Kenkyusho, Terumo Kabushiki Kaisha
• Inventors: Nakamura, Hideki, Kaneko, Masao
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Redding, David
• Application Number:
  US07/295,596
• Time in Patent Office: 1,281 Days
• Field of Search: 422/52, 422/55, 422/56, 422/88, 422/82.06-82.08, 422/86, 436/136, 436/172, 436/68, 436/138, 252/301.26, 252/408.1, 250/458.1, 250/459.1, 250/461.1, 356/41, 356/317, 356/318
• US Class Current: 422/425
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 31/221 for investigating pH value
• CPC Class Codes: G01N 31/225 for oxygen, e.g. including dissolved oxygen

Abstract

A probe for measuring oxygen concentration including an immobilized polypyridine metal complex, which can be used for measurement of oxygen concentration even in a case where the exciting ray to be used and luminescence are visible rays and which can stably be used even when it is directly immersed in a liquid to be examined without a protective membrane, which makes miniaturization of a probe possible.

Claims

1. A probe for measuring oxygen concentration by luminescence quenching, comprising a support made of a material that conducts visible light and an immobilized polypyridine metal complex film on said support, said film comprising:

a metal selected from the group consisting of ruthenium, osmium, chromium, iridium, iron, cobalt and europium;

a polypyridine ligand selected from the group consisting of bipyridine, phenanthroline, terpyridine and derivatives thereof for complexing with said metal to form the polypyridine metal complex, said polypyridine liqand having a polymerizable vinyl group; and

means for immobilizing the polypyridine metal complex in the film, wherein said means is a macromolecule prepared by polymerizing said polypyridine ligand then reacting a metal complex to form the polypyridine metal complex.

2. The probe of claim 1, wherein said metal is selected from ruthenium and osmium.

3. The probe of claim 1, wherein said metal is ruthenium.

4. The probe of claim 1, wherein the polypyridine ligand is bypyridine.

5. The probe of claim 1, wherein the polypyridine metal complex is represented by the following formula:

##STR6## wherein M is selected from the group consisting of ruthenium and osmium.

6. The probe of claim 1, wherein the macromolecule is prepared by copolymerizing a polypyridine ligand having a polymerizable vinyl group with a copolymerizable monomer and then reacting a metal complex to form the polypyridine metal complex.

7. The probe of claim 6, wherein the copolymerizable monomer is selected from the group consisting of styrene, methacrylic acid, acrylic acid and acrylonitrile.