Biotinylated oligonucleotides and reagents for preparing the same
First Claim
Patent Images
1. A compound of the formula (I):
- ##STR17## wherein A is a --CH2 --m O-- group (wherein m is an integer of 2 to
20) or a --Cn H2n --O--t group (wherein n is an integer of 2 to 6 and t is an integer of 2 to
10) and B is a group selected from the group consisting of ##STR18## and salts thereof (wherein R2 and R3 are independently selected from the group consisting of C3-10 branched alkyl groups and C1-12 unbranched alkyl groups;
R4 is selected from the group consisting of --CRR'"'"')v (X)r (CCR'"'"'--v '"'"' ##STR19## (wherein R and R'"'"' are independently selected from the group consisting of H, C3-10 branched alkyl groups, C1-10 unbranched alkyl groups, C6-10 aryl groups, C7-12 alkaryl groups and C7-12 aralkyl groups; and
X is selected from the group consisting of --O--, --S--, ##STR20## where R and R'"'"' are as defined above;
v is an integer of 1 to 6, r is an integer of 0 to I, p is an integer of 1 to 10, and q is an integer of 0 to 10, provided that 2v+r≦
12 and 2≦
r+p+q≦
12; and
Y is a phosphate protecting group)).
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Accused Products
Abstract
Reagents of the formula I ##STR1## wherein A is --CH2 --m O-- (m=2 to 20) or --Cn H2n --O--t (n=2 to 6; t=2 to 10) and B is selected from the group consisting of ##STR2## and salts thereof are useful for the preparation of 5'"'"'-biotinylated oligonucleotides.
14 Citations
4 Claims
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1. A compound of the formula (I):
- ##STR17## wherein A is a --CH2 --m O-- group (wherein m is an integer of 2 to
20) or a --Cn H2n --O--t group (wherein n is an integer of 2 to 6 and t is an integer of 2 to
10) and B is a group selected from the group consisting of ##STR18## and salts thereof (wherein R2 and R3 are independently selected from the group consisting of C3-10 branched alkyl groups and C1-12 unbranched alkyl groups;
R4 is selected from the group consisting of --CRR'"'"')v (X)r (CCR'"'"'--v '"'"' ##STR19## (wherein R and R'"'"' are independently selected from the group consisting of H, C3-10 branched alkyl groups, C1-10 unbranched alkyl groups, C6-10 aryl groups, C7-12 alkaryl groups and C7-12 aralkyl groups; and
X is selected from the group consisting of --O--, --S--, ##STR20## where R and R'"'"' are as defined above;
v is an integer of 1 to 6, r is an integer of 0 to I, p is an integer of 1 to 10, and q is an integer of 0 to 10, provided that 2v+r≦
12 and 2≦
r+p+q≦
12; and
Y is a phosphate protecting group)). - View Dependent Claims (2, 3, 4)
- ##STR17## wherein A is a --CH2 --m O-- group (wherein m is an integer of 2 to
Specification
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Current AssigneeThe Midland Certified Reagent Company Incorporated
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Original AssigneeThe Midland Certified Reagent Company Incorporated
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InventorsZhang, Guangrong, Case, Richard V.
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Primary Examiner(s)RICHTER, JOHANN R
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Application NumberUS07/658,242Time in Patent Office503 DaysField of Search548/113US Class Current548/113CPC Class CodesC07F 9/6561 containing systems of two o...C07H 21/00 Compounds containing two or...
