Enhanced nucleic acid amplification process

• US 5,130,238 A
• Filed: 08/23/1989
• Issued: 07/14/1992
• Est. Priority Date: 06/24/1988
• Status: Expired due to Term

• Current Assignee: Cangene Corporation (Emergent Biosolutions Inc.)
• Original Assignee: Cangene Corporation (Emergent Biosolutions Inc.)
• Inventors: Henderson, Graham, Malek, Lawrence T., Sooknanan, Roy, Davey, Cheryl
• Primary Examiner(s): Moskowitz, Margaret
• Assistant Examiner(s): Chambers, Scott A.
• Application Number:
  US07/397,681
• Time in Patent Office: 1,056 Days
• Field of Search: 435/6, 435/91, 536/27
• US Class Current: 435/91.21
• CPC Class Codes: C12Q 1/6832 Enhancement of hybridisation reaction
• CPC Class Codes: C12Q 1/6865 Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

This invention relates to an improved process for amplifying a specific nucleic acid sequence. The process involves synthesizing single-stranded RNA, single-stranded DNA and Double-stranded DNA. The single-stranded RNA is a first template for a first primer, the single-stranded DNA is a second template for a second primer, and the double stranded DNA is a third template for synthesis of a plurality of copies of the first template. A sequence of the first primer or the second primer is complementary to a sequence of the specific nucleic acid and a sequence of the first primer or the second primer is homologous to a sequence of the specific nucleic acid. The improvement of the amplification process involves the addition of DMSO alone or in combination with BSA, which improves the specificity and efficiency of the amplification. The amplification process may be used to increase the quantity of a specific nucleic acid sequence to allow detection, or to increase the purity of a specific nucleic acid sequence as a substitute for conventional cloning methodology.

Claims

1. A process for the amplification of a specific nucleic acid sequence, at a relatively constant temperature and without serial addition of reagents, comprising the steps of:

(A) providing a single reaction medium containing reagents comprising

(i) a first oligonucleotide primer,

(ii) a second oligonucleotide primer comprising an antisense sequence of a promoter,

(iii) a DNA-directed RNA polymerase that recognizes said promoter,

(iv) an RNA-directed DNA polymerase,

(v) a DNA-directed DNA polymerase,

(vi) a ribonuclease that hydrolyzes RNA of an RNA-DNA hybrid without hydrolyzing single- or double-stranded RNA or DNA,

(vii) ribonucleoside and deoxyribonucleoside triphosphates, and

(vi) dimethylsulfoxide; and

then

(B) providing in said reaction medium RNA comprising an RNA first template which comprises said specific nucleic acid sequence or a sequence complementary to said specific nucleic acid sequence, under conditions such that a cycle ensues wherein

(i) said first oligonucleotide primer hybridizes to said RNA first template,

(ii) said RNA-directed DNA polymerase uses said RNA first template to synthesize a DNA second template by extension of said first oligonucleotide primer and thereby forms an RNA-DNA hybrid intermediate,

(iii) said ribonuclease hydrolyzes RNA which comprises said RNA-DNA hybrid intermediate,

(iv) said second oligonucleotide primer hybridizes to said DNA second template,

(v) said DNA-directed DNA polymerase uses said second oligonucleotide primer as template to synthesize said promoter by extension of said DNA second template; and

(vi) said DNA-directed RNA polymerase recognizes said promoter and transcribes said DNA second template, thereby providing copies of said RNA first template;

and thereafter

(C) maintaining said conditions for a time sufficient to achieve a desired amplification of said specific nucleic acid sequence.

2. A process according to claim 1, wherein said RNA first template comprises said specific nucleic acid sequence and wherein step (B) comprises providing single-stranded RNA in said reaction medium such that

(i) said first oligonucleotide primer hybridizes to said single-stranded RNA,

(ii) said RNA-directed DNA polymerase uses said single-stranded RNA as a template to synthesize a DNA second template by extension of said first oligonucleotide primer and thereby forms an RNA-DNA hybrid,

(iii) said ribonuclease hydrolyses RNA which comprises said RNA-DNA hybrid,

(iv) said second oligonucleotide primer hybridizes to said DNA second template,

(v) said DNA-directed DNA polymerase uses said second oligonucleotide primer as template to synthesize said promoter by extension of said DNA second template;

and

(vi) said DNA-directed RNA polymerase recognizes said promoter and transcribes said DNA second template, thereby providing copies of said RNA first template.

3. A process according to claim 1, wherein said RNA first template comprises a sequence complementary to said specific nucleic acid sequence and wherein step (B) comprises providing single-stranded RNA in said reaction medium such that

(i) said second oligonucleotide primer hybridizes to said single-stranded RNA,

(ii) said RNA-directed DNA polymerase uses said RNA as a template to synthesize a complementary DNA by extension of said second oligonucleotide primer and thereby forms an RNA-DNA hybrid,

(iii) said ribonuclease hydrolyses RNA which comprises said RNA-DNA hybrid,

(iv) said first oligonucleotide primer hybridizes to said complementary DNA,

(v) said DNA-directed DNA polymerase uses said complementary DNA as template to synthesize said DNA second template and said promoter by extension of said first oligonucleotide primer;

and

(vi) said DNA-directed RNA polymerase recognizes said promoter and transcribes said DNA second template, thereby providing copies of said RNA first template.

4. A process according to claim 1, wherein step (B) comprises adding to said reaction medium single-stranded DNA which comprises an antisense sequence of said promoter, such that

(i) said first oligonucleotide primer hybridizes to said single-stranded DNA,

(ii) said DNA-directed DNA polymerase uses said single-stranded RNA as a template to synthesize said DNA second template and said promoter by extension of said first oligonucleotide primer;

and

(iii) said DNA-directed RNA polymerase recognizes said promoter and transcribes said DNA second template, thereby providing copies of said RNA first template.

5. A process according to claim 4, wherein step (B) comprises adding to said reaction medium and RNA-DNA hybrid comprising said single-stranded DNA, such that said ribonuclease hydrolyzes RNA which comprises said RNA-DNA hybrid.

6. A process according to claim 1, wherein step (B) comprises adding to said reaction medium single-stranded DNA which comprises said DNA second template, such that

(i) said second oligonucleotide primer hybridizes to said single-stranded DNA,

(ii) said DNA-directed DNA polymerase uses said second oligonucleotide primer as template to synthesize said promoter by extension of said DNA second template;

and

(iii) said DNA-directed RNA polymerase recognizes said promoter and transcribes said DNA second template, thereby providing copies of said RNA first template.

7. A process according to claim 6, wherein step (B) comprises adding to said reaction medium and RNA-DNA hybrid comprising said single-stranded DNA, such that said ribonuclease hydrolyzes RNA which comprises said RNA-DNA hybrid.

8. A process according to claim 2, wherein step (B) comprises adding to said reaction medium a DNA comprising said promoter, such that said DNA-directed RNA polymerase transcribes said DNA, thereby synthesizing said single-stranded RNA.

9. A process according to claim 3, wherein step (B) comprises adding to said reaction medium a DNA comprising said promoter, such that said DNA-directed RNA polymerase transcribes said DNA, thereby synthesizing said single-stranded RNA.

10. A process according to claim 1, wherein said second oligonucleotide primer further comprises an antisense sequence of a transcription initiation site for said DNA-directed RNA polymerase, said antisense sequence of said transcription initiation site being operatively linked to said antisense sequence of said promoter.

11. A process according to claim 10, wherein said DNA-directed RNA polymerase is bacteriophage T7 RNA polymerase and wherein said antisense sequence of a transcription initiation site and said antisense sequence of said promoter together comprise the nucleotide sequence

12. A process according to claim 1, wherein step (B) further comprises adding a sample to said reaction medium under conditions such that, if said sample thereby provides RNA comprising an RNA first template which comprises said specific nucleic acid sequence or a sequence complementary to said specific nucleic acid sequence, said cycle ensues, and wherein said process further comprises, after step (C), a step (D) of monitoring said reaction medium for consumption of any of said reagents (i), (ii) and (vii) or for accumulation of any product of said cycle.

13. A process according to claim 12, wherein step (D) comprises detecting a nucleic acid product of said cycle.

14. A process according to claim 13, wherein step (D) comprises detecting said nucleic acid product using a nucleic acid probe.

15. A process according to claim 13, wherein step (D) comprises detecting said nucleic acid product using restriction endonucleases and electrophoretic separation.

16. A process according to claim 13, wherein step (D) comprises monitoring the accumulation of said RNA first template.

17. A process according to claim 13, wherein step (D) comprises monitoring the accumulation of said DNA second template.

18. A process according to claim 13, wherein step (D) comprises monitoring DNA containing said promoter.

19. A process according to claim 13, wherein step (D) comprises monitoring the accumulation of said RNA-DNA hybrid intermediate.

20. A process according to claim 13, wherein step (D) further comprises comparing consumption of any reagent of said reagents (i), (ii) and (vii) or accumulation of any product of said cycle with a value representing consumption of said reagent or accumulation of said product in said reaction medium in the absence of said specific nucleic acid sequence and said sequence complementary thereto.

21. A process according to claim 1, wherein said ribonuclease comprises Escherichia coli ribonuclease H.

22. A process according to claim 1, wherein said ribonuclease comprises calf thymus ribonuclease H.

23. A process according to claim 1, wherein said first oligonucleotide primer to said second oligonucleotide primer is bound reversibly to an immobilized support.

24. A process according to claim 1, wherein said DNA-directed RNA polymerase is a bacteriophage RNA polymerase.

25. A process according to claim 24, wherein said DNA-directed RNA polymerase is bacteriophage T7 RNA polymerase.

26. A process according to claim 24, wherein said DNA-directed RNA polymerase is bacteriophage T3 polymerase.

27. A process according to claim 24, wherein said DNA-directed RNA polymerase is bacteriophage φ

II polymerase.

28. A process according to claim 24, wherein said DNA-directed RNA polymerase is Salmonella bacteriophage sp6 polymerase.

29. A process according to claim 24, wherein said DNA-directed RNA polymerase is Pseudomonas bacteriophage gh-1 polymerase.

30. A process according to claim 1, wherein said RNA-directed DNA polymerase is a retrovirus reverse transcriptase.

31. A process according to claim 30, wherein said retrovirus reverse transcriptase is avian myeloblastosis virus polymerase.

32. A process according to claim 30, wherein said retrovirus reverse transcriptase is a Moloney murine leukemia virus polymerase.

33. A process according to claim 1, wherein said DNA-directed DNA polymerase lacks exonuclease activity.

34. A process according to claim 1, wherein all DNA polymerases in said reaction medium lack exonuclease and DNA endonuclease activity.

35. A process according to claim 1, wherein said DNA-directed DNA polymerase is avian myeloblastosis virus polymerase.

36. A process according to claim 1, wherein said DNA-directed DNA polymerase is DNA polymerase α

or DNA polymerase β

.

37. A process according to claim 1, wherein said DNA directed DNA polymerase is calf thymus DNA polymerase.

38. A process according to claim 1, wherein step (C) comprises maintaining said conditions for a time between 30 minutes and 4 hours.

39. A process according to claim 1, further comprising the steps of ligating a DNA product of said cycle into a cloning vector and then cloning said DNA product.

40. A process according to claim 39, further comprising the step of expressing a product encoded by said DNA product of said cycle in an expression system.

41. A process according to claim 1, wherein said dimethylsulfoxide is provided at a final concentration in the range of 2-15%.

42. A process according to claim 41, wherein said dimethylsulfoxide is provided at a final concentration of about 15%.

43. A process according to claim 1, wherein said single reaction medium of step (A) further comprises bovine serum albumin.

44. A process according to claim 43, wherein said bovine serum albumin is provided at a final concentration in the range of 50-500 μ

g/ml.

45. A process according to claim 1, wherein said dimethylsulfoxide is provided at a final concentration of about 15% and said single reaction medium of step (A) further comprises bovine serum albumin provided at a final concentration in the range of 50-500 μ

g/ml.

46. A kit for amplifying nucleic acid sequences, comprising an assemblage of

(a) a receptacle containing a first oligonucleotide primer,

(b) a receptacle containing a second oligonucleotide primer comprising an antisense sequence of a promoter,

(c) a receptacle containing a ribonuclease that hydrolyses RNA of an RNA/DNA hybrid without hydrolyzing single- or double-stranded RNA or DNA,

(d) a receptacle containing an RNA-directed DNA polymerase,

(e) a receptacle containing an DNA-directed RNA polymerase,

(f) a receptacle containing an DNA-directed DNA polymerase,

(g) a receptacle containing ribonucleoside triphosphates,

(h) a receptacle containing deoxyribonucleoside triphosphates, and

(i) a receptacle containing dimethylasulfoxide.

47. A kit according to claim 46, further comprising a receptacle containing bovine serum albumin.