Calibration solution and calibration method for sensor

• US 5,132,000 A
• Filed: 09/22/1989
• Issued: 07/21/1992
• Est. Priority Date: 09/24/1988
• Status: Expired due to Fees

• Current Assignee: Terumo Kabushiki Kaisha
• Original Assignee: Terumo Kabushiki Kaisha
• Inventors: Sone, Atsushi, Ushizawa, Norihiko, Shimomura, Takeshi
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Bell, Bruce F.
• Application Number:
  US07/411,114
• Time in Patent Office: 1,033 Days
• Field of Search: 204/153.1, 204/400, 204/153.21, 204/416, 436/18, 436/8, 436/10, 436/7
• US Class Current: 205/787.5
• CPC Class Codes: A61B 5/14539 for measuring pH
• CPC Class Codes: A61B 5/1495 Calibrating or testing of in-vivo probes
• CPC Class Codes: G01N 2496/70 Blood gas control solutios containing dissolved oxygen, bicarbonate and the like
• CPC Class Codes: G01N 27/4165 for pH meters
• CPC Class Codes: G01N 33/96 involving blood or serum control standard

Abstract

A calibration solution, which is used when measuring the ion concentration of solution under examination such as blood or other body fluids using a pH sensor or the like ion sensor with a solid electrode, is prepared by adding NaCl to a standard buffer solution and thus setting the ionic strength of the solution to be substantially identical with the ionic strength of solution under examination. A sensor calibration method is also disclosed, in which at least two reference calibration solutions having different pH values are prepared, the electromotive force of the sensor in these calibration solutions is measured by immersing the sensor in the solutions, and a calibration formula is produced from the electromotive force for the pH values of the reference calibration solutions. A predetermined amount of bicarbonate buffer solution is added to the calibration solution to maintain a substantially constant ionic strength of the solution, thus permitting simultaneous calibration of an ion sensor and a gas sensor.

Claims

1. A calibration solution for a sensor with a solid electrode for measuring ion concentration of a solution under examination, comprising a standard buffer solution which comprises a mixture solution of Na₂ HPO₄ and NaH₂ PO₄ in a 1 to 1 ratio, and with NaCl being added to said buffer solution to provide an ionic strength substantially identical with the ionic strength of said solution under examination.

2. A calibration solution for a sensor with a solid electrode for measuring ion concentration of a solution under examination, comprising a standard buffer solution which comprises a mixture solution of Na₂ HPO₄ and NaH₂ PO₄ in a 4 to 1 ratio, and with NaCl being added to said buffer solution to provide an ionic strength substantially identical with the ionic strength of said solution under examination.

3. A calibration solution for a sensor with a solid electrode for measuring ion concentration of a solution under examination, comprising a standard buffer solution, and with NaCl being added to said buffer solution to provide an ionic strength in the range of 0.08 to 0.18 which is substantially identical with the ionic strength of said solution under examination.

4. A calibration method for calibrating an ion sensor comprising the steps of:

setting an ionic strength which is substantially identical with the ionic strength of a solution under examination by adding NaCl to a standard buffer solution which comprises a mixture of Na₂ HPO₄ and NaH₂ PO₄ in a 1 to 1 ratio while preparing at least two reference calibration solutions having different pH values;

measuring the electromotive force in each of said reference calibration solutions by immersing said sensor in each of said solutions; and

producing a calibration formula from the electromotive forces with respect to the pH values of each of said reference calibration solutions.

5. A calibration method for calibrating an ion sensor comprising the steps of:

setting an ionic strength which is substantially identical with the ionic strength of a solution under examination by adding NaCl to a standard buffer solution which comprises a mixture of Na₂ HPO₄ and NaH₂ PO₄ in a 4 to 1 ratio while preparing at least two reference calibration solutions having different pH values;

measuring the electromotive force in each of said reference calibration solutions by immersing said sensor in each of said solutions; and

producing a calibration formula from the electromotive forces with respect to the pH values of each of said reference calibration solutions.

6. A calibration solution for a sensor with a solid electrode for measuring the ion concentration of a solution under examination which comprises a standard buffer solution comprised mainly of a phosphate buffer which buffer is a mixture composed of Na₂ HPO₄ and NaH₂ PO₄, and with NaCl being added to said buffer solution to permit the solid membrane surface of said electrode to be held at equilibrium potential.

7. A calibration solution for a sensor for simultaneously calibrating an ion sensor and a gas sensor, these sensors being provided with solid electrodes with respect to the ion concentration and dissolved gas concentration in solution under examination;

NaCl being added to said standard buffer solution to provide an ionic strength substantially identical with the ionic strength of solution under examination, a predetermined quantity of bicarbonate buffer solution being added to maintain the ion concentration substantially constant.

8. The calibration solution for a sensor according to claim 7, wherein said bicarbonate buffer solution is NaHCO₃.

9. The calibration solution according to claim 8, which contains predetermined quantities of carbon and oxygen gases.

10. The calibration solution according to claim 9, wherein said sensor is a carbon dioxide gas sensor.

11. The calibration solution for a sensor according to claim 9, wherein said sensor is an oxygen gas sensor.

12. A calibration method for simultaneously calibrating an ion sensor and a gas sensor comprising the steps of:

preparing at least two standard buffer solutions having different pH values and partial gas pressure values by adding NaCl for setting an ionic strength substantially identical with the ionic strength of solution under examination and also adding a bicarbonate buffer solution for stabilizing the pH value;

measuring the electromotive forces of said ion and gas sensors in said reference calibration solutions by immersing said sensors in said solutions; and

forming calibration formulas by plotting the electromotive forces of said ion gas sensors for the pH values and partial gas pressure values of said standard buffer liquids, respectively.

13. The calibration method according to claim 12, wherein said ion and gas sensors are respectively a pH sensor and a carbon dioxide gas sensor.

15. The calibration method according to claim 12, wherein said bicarbonate buffer solution is NaHCO₃.

14. A calibration method for simultaneously calibrating a pH sensor, a carbon dioxide gas sensor and an oxygen gas sensor comprising the steps of:

preparing at least two standard buffer solutions having different pH values, partial carbon dioxide gas pressure values and partial oxygen pressure values by adding NaCl for setting an ionic strength substantially identical with the ionic strength of solution under examination and also adding a bicarbonate buffer solution for stabilizing the pH value;

measuring the electromotive forces of said pH and carbon dioxide gas sensors and also the current in said oxygen sensor in said reference calibration solutions by immersing said sensors in said solutions; and

forming calibration formulas by plotting the electromotive forces of said pH and carbon gas sensors for the pH values and partial carbon dioxide gas pressure values of said reference calibration solutions and also forming a calibration formula by plotting the current value of said oxygen sensor for the partial oxygen pressure value.