Apparatus for measuring blood constituent counts

• US 5,132,087 A
• Filed: 10/16/1989
• Issued: 07/21/1992
• Est. Priority Date: 10/16/1989
• Status: Expired due to Fees

• Current Assignee: Robert A. Levine, Stephen C. Wardlaw
• Original Assignee: Robert A. Levine, Stephen C. Wardlaw
• Inventors: Kristen L. Manion, Wardlaw, Stephen C., Levine, Robert A.
• Primary Examiner(s): Hill, Jr., Robert J.
• Application Number:
  US07/421,639
• Time in Patent Office: 1,009 Days
• Field of Search: 436/70, 436/808, 436/810, 422/61, 73/61.4, 73/61.1 R, 73/865.9, 356/39, 350/526, 210/744
• US Class Current: 356/39
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: G01N 15/05 in blood
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence

Abstract

Blood constituents are separated by centrifugation in a transparent tube which contains a float for physically elongating certain of the constituents. The centrifuged sample in the tube is positioned on a layer measuring device with which red cell and buffy coat constituent band heights can be measured in ambient light. The buffy coat constituent measurements are made under optical magnification. The measuring device has a mathematically derived red cell layer nomogram printed thereon, and a separate scale for measuring buffy coat constituent bands. Conversion tables are provided for converting the measured buffy coat constituent band lengths into constituent cell counts. The measuring device includes tube-engaging portions for properly positioning the tube during the measurements, and the red cell scale automatically compensates for the presence of the float and densification of red cells needed for proper banding.

Claims

1. A device for use in measuring blood cell constituent counts in a sample of centrifuged blood contained in a transparent centrifuge tube having a constituent buffy coat layer-elongating float in the blood sample, said device comprising:

a) a plaque for supporting the centrifuge tube;

b) a stop surface formed on one surface of said plaque, said stop surface being operable to engage one end of said centrifuge tube; and

c) mathematically derived nomogram indicia means printed on said plaque adjacent said stop surface, said nomogram indicia means including a red cell layer nomogram scale and a separate nomogram scale for measuring at least one buffy coat constituent band, said nomogram scales being operable to provide red cell and buddy coat constituent band layer readings from a sample of blood in the centrifuge tube when the latter is disposed on said plaque, said nomogram scales being adjusted to account for the presence of the float in the blood sample;

d) conversion table means on said plaque for converting observed buffy coat constituent band layer readings into buffy coat constituent counts; and

e) a plurality of surface corrugations on said plaque, said corrugations extending from said stop surface across said nomogram indicia means, and said corrugations providing a plurality of adjacent parallel grooves operable to properly position a centrifuge tube in a plurality of different locations along said nomogram indicia means.

2. The device of claim 1 wherein said red cell layer nomogram scale is adjusted to account for the presence of a red cell densifying reagent in the blood sample.