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Polymerization and copolymerization of proteins

  • US 5,134,071 A
  • Filed: 02/06/1989
  • Issued: 07/28/1992
  • Est. Priority Date: 02/06/1989
  • Status: Expired due to Fees
First Claim
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1. A method for producing a polyprotein having at least 10 units held together by sulfur to sulfur or sulfur to carbon bonds, each unit comprising a protein and one or more heterobifunctional reagents capable of forming a covalent bond with an amino group wherein at least one of the heterobifunctional reagents is capable of forming a covalent sulfur-sulfur or carbon-sulfur bond with a thiol group and at least one of the reagents has a thiol group or a group capable of being converted into a thiol group, the method comprising the steps of:

  • a) forming a first protein-heterobifunctional reagent complex by treating a first protein with a sufficient amount of a heterobifunctional reagent so as to form a covalent bond between amino groups of the first protein and 3 to 5 molecules of the reagent per protein molecule;

    b) forming a second protein-heterobifunctional reagent complex by treating a second protein with a sufficient amount of a heterobifunctional reagent so as to form a covalent bond between amino groups of the second protein and at least 5 molecules of the reagent per protein unit;

    c) providing a thiol group on at least one of the reagents; and

    d) joining the first protein-heterobifunctional reagent complex and the second protein-heterobifunctional reagent complex by forming sulphur-sulphur or carbon-sulphur bonds into a polyprotein having at least 10 units.

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