Mosaic microcolumns, slabs, and separation media for electrophoresis and chromatography
First Claim
1. A composition for use in chromatographic and electrophoretic separations of molecules comprisingdispersoids,a solution immiscible with the dispersoids containing chemical monomers which, in the presence of crosslinking agent and initiator, polymerize to form a continuous polymeric matrix incorporating the dispersoids, wherein the separation properties of the dispersoids are different from the separation properties of the polymeric matrix,a crosslinking agent and initiator.
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Abstract
A method and compositions for separating molecules based on molecular size, shape, affinity, chirality, weight, charge, and hydrogen bonding, using a mosaic matrix formed by polymerizing a dispersion of dispersoids within a polymeric matrix. The dispersoids and matrix can be of the same or different hydrophobicity or hydrophilicity. The dispersoids can be porous or non-porous. The mosaic matrix can be used with existing chromatographic and electrophoresis apparatus to effect an enhanced separation of molecules, particularly of nucleic acids and peptides, by application of a solution and/or an electrical field to the matrix. The solution can form a pH, ionic, or composition gradient, and be applied using gravity or under pressure. The electrical field can be continuous, pulsed, or two-dimensional.
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Citations
33 Claims
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1. A composition for use in chromatographic and electrophoretic separations of molecules comprising
dispersoids, a solution immiscible with the dispersoids containing chemical monomers which, in the presence of crosslinking agent and initiator, polymerize to form a continuous polymeric matrix incorporating the dispersoids, wherein the separation properties of the dispersoids are different from the separation properties of the polymeric matrix, a crosslinking agent and initiator.
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12. A method for providing a composition for separation of molecules comprising
mixing dispersoids, a solution immiscible with the dispersoids containing chemical monomers which, in the presence of crosslinking agent and initiator, polymerize to form a continuous polymeric matrix incorporating the dispersoids, wherein the separation properties of the dispersoids are different from the separation properties of the polymeric matrix, crosslinking agent and initiator, and polymerizing the monomers to form a continuous matrix containing the dispersoids within the matrix.
Specification