Chromatography stationary phase material for high performance liquid chromatography

• US 5,135,650 A
• Filed: 05/02/1990
• Issued: 08/04/1992
• Est. Priority Date: 12/22/1988
• Status: Expired due to Term

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Hjerten, Stellan, Liao, Jia-Li
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US07/518,038
• Time in Patent Office: 825 Days
• Field of Search: 536/18.3, 536/120, 210/635, 210/656, 210/198.2, 210/502.1, 502/404, 502/439, 530/413, 530/417
• US Class Current: 210/198.2
• CPC Class Codes: B01J 20/262 obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G
• CPC Class Codes: B01J 20/267 Cross-linked polymers
• CPC Class Codes: B01J 20/28054 characterised by their surface properties or porosity
• CPC Class Codes: B01J 20/28097 being coated, filled or plugged with specific compounds
• CPC Class Codes: B01J 20/281 Sorbents specially adapted for preparative, analytical or investigative chromatography
• CPC Class Codes: B01J 20/285 based on polymers
• CPC Class Codes: B01J 20/286 Phases chemically bonded to a substrate, e.g. to silica or to polymers
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3278 Polymers being grafted on the carrier

Abstract

Highly compressible chromatographic stationary phase particles such as agarose beads are made rigid to a degree suitable for use in HPLC, and nonporous to proteins by one of two procedures. The first involves shrinking the beads with the use of an organic solvent in which the agarose bead will neither dissolve nor swell to collapse the porosity, followed by crosslinking the bead surfaces inside the collapsed pores to fix the pores in their collapsed state. The second involves filling the pores (without shrinkage of the beads) with a polymerizable substance which grafts to the pore surface, and performing the graft polymerization. The invention also extends to rigid beads, which are rendered deformable to a limited degree by coating the surface with a polymer. Finally, porous rigid beads are rendered nonporous by polymerizing a polymerizable material inside the pores in the same manner as the porous compressible beads.

Claims

1. A method for converting agarose beads with pores filled with an aqueous medium and of sufficient porosity to be permeable to proteins, to beads of sufficiently low porosity to be substantially impermeable to proteins, said method comprising:

(a) treating said agarose beads with a first organic solvent in which agarose is soluble but which promotes swelling of agarose, to weaken the agarose while retaining said agarose in the form of beads;

(b) replacing substantially all of said first organic solvent and any remaining water in said agarose beads with a second organic solvent which neither dissolves nor swells the agarose to cause the pores of said agarose beads to at least partially collapse, thereby imparting to said beads a reduced porosity;

(c) placing an agarose crosslinking agent inside the pores of said beads; and

(d) causing said crosslinking agent to crosslink the agarose inside said pores to fix said reduced porosity.

2. A method in accordance with claim 1 in which said crosslinking agent is a member selected from the group consisting of epoxides, bisepoxides and trisepoxides.

3. A method in accordance with claim 1 in which said crosslinking agent is a member selected from the group consisting of 1,4-butanediol diglycidyl ether and γ

-glycidoxypropyltrimethoxy silane.

4. A method in accordance with claim 1 in which said second organic solvent is one tending to avoid aggregation of agarose beads.

5. A method in accordance with claim 1 in which said first organic solvent is water-miscible, and miscible with said second organic solvent.

6. A method in accordance with claim 1 in which said agarose beads having an average bead diameter ranging from about 3 microns to about 300 microns.

7. A method in accordance with claim 1 in which said agarose beads having an average bead diameter ranging from about 10 microns to about 100 microns.

8. A method in accordance with claim 1 in which said agarose beads have an agarose concentration ranging from about 8% to about 20% by weight.

9. A method in accordance with claim 1 in which said agarose beads have an agarose concentration ranging from about 10% to about 15% by weight.

10. A method in accordance with claim 1 in which said reduced porosity is sufficiently reduced to render the agarose beads resulting from step (c) nonpermeable to proteins of molecular weight 12,000 and higher.

11. A method in accordance with claim 1 in which said reduced porosity is sufficiently reduced to render the agarose beads resulting from step (c) nonpermeable to proteins of molecular weight 10,000 and higher.

12. A method in accordance with claim 1 in which said reduced porosity is sufficiently reduced to render the agarose beads resulting from step (c) nonpermeable to proteins of molecular weight 3,000 and higher.