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Direct measurement of HDL cholesterol via dry chemistry strips

DC
  • US 5,135,716 A
  • Filed: 02/25/1991
  • Issued: 08/04/1992
  • Est. Priority Date: 07/12/1989
  • Status: Expired due to Term
First Claim
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1. A device for determining HDL cholesterol by obtaining plasma from whole blood and determining HDL level from the plasma which comprises:

  • (a) a physical transport medium;

    (b) a microporous plasma separation membrane having a first surface against said physical transport medium and having a second surface opposite and away from said physical transport medium;

    (c) a filtering membrane having a first surface and a second surface and being capable of filtering LDL and VLDL precipitates from a blood or plasma flow containing these, said filtering membrane having its first surface in contact with said microporous plasma separation membrane;

    (d) at least one plasma collecting test membrane in contact with a portion of said second surface of said filtering membrane and having one or more reactants therein capable of reacting with said plasma to display at least one characteristic of HDL cholesterol; and

    ,(e) LDL and VLDL reactants to form LDL and VLDL precipitates, said reactants being located in one or more of said microporous plasma separation membrane, said filtering membrane and said physical transport medium.

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