Process for the determination by means of free radicals of the antioxidant properties of a living organism or a potentially aggressive age

• US 5,135,850 A
• Filed: 09/17/1990
• Issued: 08/04/1992
• Est. Priority Date: 01/27/1989
• Status: Expired due to Term

• Current Assignee: Spiral Recherche et Developpment
• Original Assignee: Spiral Recherche et Developpment
• Inventors: Prost, Michel
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): WILLIAMS, JANE
• Application Number:
  US07/576,460
• Time in Patent Office: 687 Days
• Field of Search: 435/29, 435/240.1, 436/63
• US Class Current: 435/29
• CPC Class Codes: G01N 33/5014 for testing toxicity
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention relates to a novel method of assaying or evaluating the antioxidizing activities of a living organism or a potentially aggressive agent, said method, which comprises using free radicals as a means of inducing cell lysis, being characterized in that

1) a free radical generator is brought into contact, in an appropriate liquid biological medium, with a cell material selected from the group consisting of

(a) human, animal and plant cells,

(b) fragments of said cells, and

(c) synthetic walls and fragments thereof containing liposomes, said cell material having first been contaminated with a potentially aggressive agent;

2) the release of free radicals from said free radical generator is induced; and

3) the lysis of the cell material by the free radicals is evaluated by comparison with a control containing said cell material which has not been contaminated.

Claims

1. A method of assaying or evaluating the effect on lysis of a cell material which has been subjected to a chemical or physical agent comprising:

1) a free radical generator is brought into contact, in an appropriate liquid biological medium, with a cell material selected from the group consisting of

(a) human, animal and plant cells,

(b) fragments of said cells, and

(c) liposomes,

said cell material having first been contacted with a chemical or physical agent;

2) the release of free radicals from said free radical generator is induced; and

3) evaluating the lysis of the cell material as compared to a control sample.

2. A method according to claim 1, wherein the cell material is selected from the group consisting of pigmented cells of human, animal or vegetable origin.

3. A method according to claim 2, wherein said pigmented cells are erythrocytes of human or animal origin.

4. A method according to claim 1, wherein the cell material is a synthetic wall fragment.

5. A method according to claim 4, wherein the cell material is a liposome material.

6. A method according to claim 1, wherein said free radical generator is selected from the group consisting of products which release free radicals according to a 0 or 1st order kinetics.

7. A method according to claim 1, wherein said free radical generator is selected from the group consisting of products which release oxidizing free radicals.

8. A method according to any one of claims 1, 6 or 7, wherein said free radical generator is 2,2'"'"'-azo-bis(2-amidinopropane) dihydrochloride.

9. A method according to claim 1, wherein 50 to 200 mmol/l of free radical generator are brought into contact with a cell material at a concentration of 10 to 20% w/v in an aqueous biological medium.

10. A method according to claim 1 or claim 9, wherein the free radicals are generated by incubation at 37°

C.