Compositions and methods for determining the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine

• US 5,135,863 A
• Filed: 12/23/1988
• Issued: 08/04/1992
• Est. Priority Date: 12/23/1988
• Status: Expired due to Term

• Current Assignee: Dade Behring Marburg GmbH (Siemens AG)
• Original Assignee: Syntec Incorporated (Atos SE)
• Inventors: Hu, Mae Wan Leng, Ullman, Edwin F., Lin, Cheng-I, Pirio, Marcel, Freeman, James V., Hsu, Chen-jung
• Primary Examiner(s): Naf, David M.
• Assistant Examiner(s): Weber, Jon P.
• Application Number:
  US07/290,487
• Time in Patent Office: 1,320 Days
• Field of Search: 435/188, 435/964, 435/94.1, 435/94.4, 435/7, 435/9, 436/532, 564/162, 564/164, 564/165, 564/381, 530/404
• US Class Current: 435/188
• CPC Class Codes: C07C 233/18 having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
• CPC Class Codes: C07C 323/12 the carbon skeleton being acyclic and saturated
• CPC Class Codes: C07C 323/32 having at least one of the nitrogen atoms bound to an acyclic carbon atom of the carbon skeleton
• CPC Class Codes: C07C 323/41 Y being a hydrogen or an acyclic carbon atom
• CPC Class Codes: G01N 33/535 with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
• CPC Class Codes: G01N 33/946 CNS-stimulants, e.g. cocaine, amphetamines
• CPC Class Codes: Y10S 435/964 including enzyme-ligand conjugate production, e.g. reducing rate of nonproductive linkage
• CPC Class Codes: Y10S 436/816 Alkaloids, amphetamines, and barbiturates
• CPC Class Codes: Y10S 436/822 Identified hapten

Abstract

The instant invention is directed toward an immunoassay which can determine the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine by employing at least two conjugates, each comprised of a functionally similar label bound to an amphetamine analog and a methamphetamine analog respectively and an antibody to amphetamine and an antibody to methamphetamine wherein at least one of the antibodies is a monoclonal antibody.

Claims

1. A compound of the formula:

##STR8## wherein R is hydrogen or methyl;

X is oxygen, sulfur or is a bond;

R₁ is alkylene of 1 to 6 carbon atoms;

R₂ is alkylene of 2 to 6 carbon atoms;

n is 0 or 1;

R₃ is hydrogen, --SR₄ wherein R₄ is alkyl of from 1 to 6 carbon atoms, or is (A)_p Z wherein A is derived from a functionality capable of reacting with a thiol group to form a bond between the sulfur atom of the thiol group and A, and Z is a poly(amino acid);

p is 1 when R₃ is hydrogen or --SR₄ and is a number from 1 to the molecular weight of the poly(amino acid) divided by 500 when R₃ is (A)_p Z with the proviso that when n is zero then R₁ is alkylene of 2 to 6 carbon atoms and with the further proviso that the benzene ring is bonded at the meta or the para position to the ##STR9## and when X is a bond, the benzene ring is bonded at the para position.

2. A compound according to claim 1 wherein Z is an enzyme.

3. A compound according to claim 2 wherein said enzyme is glucose-6-phosphate dehydrogenase.

4. A compound according to claim 1 wherein X is oxygen;

R₁ is methylene;

n is 1; and

R₂ is ethylene.