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Differential separation assay

  • US 5,137,609 A
  • Filed: 01/31/1992
  • Issued: 08/11/1992
  • Est. Priority Date: 01/31/1992
  • Status: Expired due to Fees
First Claim
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1. A method for detecting target substances during electrophoresis comprising,mixing a specific binding agent with a sample which may contain a target substance, the binding agent being specific to the target substance and having a fluorescent characteristic, the amount of binding agent in excess of what will react with the target substance so that there is free and bound binding agent in the sample, both the bound and free binding agent having expected electrophoretic migration times from a starting location to a measuring place,conducting electrophoresis of said mixture in a path defined by electrodes,measuring and recording the times wherein substances having said fluorescent characteristic reach the measuring place,searching said recorded times for bound binding agent in relation to free binding agent using said expected electrophoretic migration times in comparison to said measured times wherein finding of said bound binding agent indicates presence of said target substance.

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