Method and device for cytology and microbiological testing
First Claim
1. An apparatus for collecting cellular components and biological specimens from a fresh unpreserved body fluid for cytology and pathogen testing comprising a syringe, a plurality of specimen collection containers stacked together in fluid communication with said syringe, each specimen collection container comprising a two-piece housing comprising a male member and a female member removably mounted together, a membrane filter means is mounted in said male member adjacent a housing chamber defined by said male member, said membrane filter means allowing biologicaI fluid flow therethrough while concentrating biological components on said membrane filter means, said membrane filter means being easily accessible for removal of the biological components collected thereon.
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Accused Products
Abstract
The present invention is directed towards an apparatus and a method which can use immunoassay in sample treatment apparatus for diagnostic and testing purposes of dialysis fluid or urine by concentrating the urinary sediments on a first membrane and concentrating bacteria on a second membrane. Urine is transported through the tubular container under pressure to flow through the sample container which separately screens off the urinary sediments and the bacteria for collection and testing.
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Citations
33 Claims
- 1. An apparatus for collecting cellular components and biological specimens from a fresh unpreserved body fluid for cytology and pathogen testing comprising a syringe, a plurality of specimen collection containers stacked together in fluid communication with said syringe, each specimen collection container comprising a two-piece housing comprising a male member and a female member removably mounted together, a membrane filter means is mounted in said male member adjacent a housing chamber defined by said male member, said membrane filter means allowing biologicaI fluid flow therethrough while concentrating biological components on said membrane filter means, said membrane filter means being easily accessible for removal of the biological components collected thereon.
- 13. An apparatus for collecting bacteria and urinary sediments from a human fluid sample comprising a pump means, a plurality of specimen collection containers removably mounted to each other while allowing fluid passage therethrough, one of said specimen collection containers being mounted to said pump means, each of said specimen collection containers comprising a housing provided with connector means allowing the housing to be connected to other specimen collection containers, said housing comprising two mateable sections, one of said housing sections defining a chamber, a filter membrane seat defined in said one housing section positioned adjacent said chamber and a filter membrane mounted in said membrane seat, the other housing section being removably mounted to the first housing section so that said housing sections can be opened for access to the filter membrane, said other housing section including filter membrane positioning means which keeps said filter membrane in position on said filter membrane seat and defining a throughgoing bore which is adapted to hold and seat a needle, each filter membrane which is downstream of the previous filter membrane having a smaller pore size then the prior upstream filter membrane with at least one of said filter membranes retaining cellular sediments from said human fluid sample and at least one of said filter membranes retaining bacteria from said human fluid sample.
- 23. An apparatus for testing for cellular specimens and bacteria in a biological fluid comprising a pump means, a plurality of specimen collection units serially mounted to said pump means for selective removal from each other and said pump means, each said specimen collection unit comprising a separable sectional housing with an inlet and outlet means, said inlet and outlet means additionally serving as connection means with other specimen collection units, a filter membrane means mounted in said housing in one of said housing sections allowing fluid flow therethrough by said biological fluid while selectively retaining either cellular specimens or bacteria dependent upon the pore size of the membrane filter, each filter membrane means in each specimen collection unit has a larger porosity than the filter membrane means in the next downstream specimen collection container.
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25. A method of testing for collecting different biological specimens from biological fluid comprising the steps of:
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a. collecting a biological fluid into a container; b. passing the biological fluid through a series of separate removably connected sectioned filter containers causing different preselected biological components in the biological fluid to be deposited on membrane filter means held in each sectioned filter container with one of said sectioned filter containers trapping cellular components from the biological fluid; c. removing one of the containers which traps the cellular components from the series of separate connected removable sectioned filter containers and opening the filtered sectioned container to provide access to the membrane filter means with trapped cellular components contained therein; and d. applying the filter membrane surface to a slide depositing cells on said slide. - View Dependent Claims (26, 27)
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28. A method of testing for collecting different biological specimens from human body fluid comprising the steps of:
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a. collecting a human body fluid into a container; b. passing the human body fluid through a series of separate removably connected sectioned filter containers causing different preselected biological components in the human body fluid to be deposited on membrane filter means held in each sectioned filter container with one of said sectioned filter containers trapping cellular components from the human body fluid; c. removing one of the containers which traps the cellular components from the series of separate connected removable sectioned filter containers and opening the filtered sectioned container to provide access to the membrane filter means with trapped cellular components contained therein; and d. applying the filter membrane surface to a slide depositing fresh unpreserved cells on said slide. - View Dependent Claims (29)
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30. A method of testing for predetermined biological specimens in a biological fluid comprising the steps of:
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a. collecting a biological fluid into a container; b. passing the biological fluid through a series of separate removably connected sectioned filter containers causing different preselected biological components in the biological fluid to be deposited on membrane filter means held in each sectioned filter container with one of said sectioned filter containers trapping bacteria from the biological fluid; c. removing the container which traps bacteria from the series of separate connected removable sectioned filter containers and opening the filtered sectioned container to provide access to the membrane filter means with trapped bacteria contained therein; d. eluting the trapped bacteria from the membrane filter means into a microbiological culture plate; and e. incubating the bacteria into an identifiable bacterial colony.
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31. A method of testing for predetermined biological specimens in a biological fluid comprising the steps of:
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a collecting a biological fluid into a container; b. passing the biological fluid through a series of separate removably connected sectioned filter containers causing different preselected biological components in the biological fluid to be deposited on membrane filter means held in each sectioned filter container with one of said sectioned filter containers trapping bacteria from the biological fluid; c. removing the container which traps bacteria from the series of separate connected removable sectioned filter containers and opening the filtered sectioned container to provide access to the membrane filter means with trapped bacteria contained therein; d. eluting the trapped bacteria into a Qualture capsule; and e. incubating the bacterial in the Qualture capsule into an identifiable bacterial colony. - View Dependent Claims (32)
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33. A method of testing for predetermined biological specimens in a preserved biological fluid comprising the steps of:
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a. collecting a preserved biological fluid into a container; b. passing the preserved biological fluid through a series of separate removably connected sectioned filter containers causing different preselected biological components in the preserved biological fluid to be deposited on membrane filter means held in each sectioned filter container with one of said sectioned filter containers trapping bacteria from the preserved biological fluid; c. removing the container which traps bacteria from the series of separate connected removable sectioned filter containers and opening the filtered sectioned container to provide access to the membrane filter means with trapped bacteria contained therein; d. eluting the trapped bacteria from the membrane filter means onto a slide; and e. microscopically examining the deposited bacteria on said slide.
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Specification