Method and apparatus for high security crypto-marking for protecting valuable objects

• US 5,139,812 A
• Filed: 06/29/1990
• Issued: 08/18/1992
• Est. Priority Date: 07/07/1989
• Status: Expired due to Fees

• Current Assignee: Bioprobe Systems
• Original Assignee: Bioprobe Systems
• Inventors: Lebacq, Philippe
• Primary Examiner(s): BELL, JANYCE A
• Application Number:
  US07/545,607
• Time in Patent Office: 781 Days
• Field of Search: 427/7, 427/145, 427/338, 427/337, 283/73, 283/74, 283/95, 283/98, 283/100, 118/31.5, 118/201
• US Class Current: 427/7
• CPC Class Codes: B41M 3/14 Security printing securities B42D25/29
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2563/185 Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
• CPC Class Codes: D21H 21/46 Elements suited for chemical verification or impeding chemical tampering, e.g. by use of eradicators
• CPC Class Codes: G06N 3/123 DNA computing
• CPC Class Codes: G07D 7/06 using wave or particle radiation
• CPC Class Codes: G07D 7/14 using chemical means
• CPC Class Codes: G09F 3/00 Labels, tag tickets, or similar identification or indication means medals or badges A44C3/00; making labels B31D1/02; sheets temporarily attached together B42F; labelling B65C; labels on record carriers G11B23/38, G11B23/40; Seals; Postage or like stamps

Abstract

A high security crypto-marking method for protecting valuable objects in which a target chemical compound suitable for subsequent detection by an appropriate detection means is applied to an object to be marked, wherein the method comprises the following operations:

a) preparing a solution of a target nucleic acid having a sequence selected to form a marking pattern and possessing a selected degree of fluidity;

b) incorporating a chosen quantity of the solution in each object of a class of objects to be marked;

thereby making it possible, subsequently, and should the occasion arise, to perform the following operation:

c) identifying or authenticating the object by using means for detecting the nucleic acid in order to reveal the marking.

The invention can be used, where required, to provide irrefutable proof of ownership of an object in the event of theft, resale, or forgery.

Claims

1. A high security crypto-marking method for protecting valuable objects in which a target chemical compound suitable for subsequent detection by an appropriate detection means is applied to an object to be marked, wherein the method comprises the following operations:

a) preparing a solution of a target nucleic acid having a known nucleotide sequence and possessing a selected degree of fluidity;

b) incorporating a chosen quantity of said solution in each object of a class of objects to be marked; and

c) identifying or authenticating the object by bringing a probe means including a chemical agent reactive with said target nucleic acid into contact with said target nucleic acid, and detecting a reaction between said agent and said target nucleic acid and/or a reaction product.

2. A method according to claim 1, wherein operation b) comprises applying a solution of nucleic acid on a surface of a medium in order to impregnate it with said solution, and subsequently in incorporating the medium impregnated in this way in each object that is to be marked.

3. A method according to claim 2, wherein the medium is made of a substance selected from the group consisting of nitrocellulose, nylon, nylon which is positively charged, paper, card, wood, plastic material, cloth, an organic substance in the form of beads or of a gel, and an inorganic substance.

4. A method according to claim 2, wherein the support is a membrane of small size suitable for being inserted flat in a thin object or suitable for being rolled up to form a roll suitable for insertion in a thick object.

5. A method according to claim 1, wherein operation b) comprises applying the solution of nucleic acid directly on a surface of the object to be marked, wherein the substance from which said surface is made enables said nucleic acid to adhere directly thereto.

6. A method according to claim 1, wherein during the operation b), the nucleic acid solution is applied in the form of a mark by writing at least one element selected from the group consisting of a number, a letter, a word, a phase, and a signature.

7. A method according to claim 1, wherein said chemical agent comprises a single strand nucleic acid whose sequence is complementary to that of the target nucleic acid incorporated in the object, which single strand nucleic acid is capable of forming a hybrid with the target nucleic acid, thereby enabling the target nucleic acid to be specifically detected by molecular hybridization.

8. A method according to claim 7, wherein the hybrid is detected by a method selected from the group consisting of radioactive compounds, non-radioactive compounds, direct detection means, indirect detection means, chemical means, and physical means.

9. A method according to claim 1, wherein the target nucleic acid and the chemical agent are selected from the group consisting of DNA and RNA of natural and of synthetic origin, from synthesized derivatives containing at least in part at least one of rare bases and modified bases, from nucleic sequences and genomes suitable for generating polymorphism, and from genome sequences and entire genomes of human beings.

10. A method according to claim 9, wherein the target nucleic acid and the chemical agent are complementary oligonucleotides whose sequences are obtained randomly.

11. A method according to claim 7, comprising determining base contents of cytosine and quanine for a randomly generated target nucleic acid and performing hybridization under specific conditions depending on the cytosine and guanine base content of the target nucleic acid sequence as randomly generated.

12. A method according to claim 1, wherein the target nucleic acid solution contains a buffer suitable for conserving the nucleic acid and for ensuring that the solution is sufficiently fluid to enable it to be applied, and wherein applicator means are used for spreading said solution and forming symbols on a surface to be marked.

13. A method according to claim 1, wherein said chemical agent is non-specific for said target nucleic acid.

22. A method according to claim 3, wherein said inorganic substance is metal, glass, ceramics, or earthenware.

23. A method according to claim 8, wherein said physical means is electrical means, electronic means, radio means or magnetic means.

24. A method according to claim 10, wherein said sequences are obtained randomly by means of a computer program.

14. A kit for high security crypto-marking for protecting valuable objects, said kit comprising:

a solution of a target nucleic acid having a known nucleotide sequence and possessing a selected degree of fluidity;

applicator means suitable for spreading said solution on a surface to be marked; and

probe means comprising a chemical agent reactive with said target nucleic acid and means for detecting a reaction between the target nucleic acid and chemical agent and/or a reaction product,

wherein said solution contains a buffer for conserving the target nucleic acid and for conferring to the solution a degree of fluidity sufficient for use in said applicator means.

15. Kit according to claim 14, wherein the concentration of target nucleic acid in the solution is such that the applicator means deposits 10 pg to 10 ng of nucleic acid per mm² of surface to be marked.

16. Kit according to claim 14, wherein the applicator means is constituted by an implement selected from the group a ballpoint pen, a nib pen, a felt tip pen, a paint-brush, a drawing instrument, and an automated machine.

17. Kit according to claim 14, further including a medium constituting a surface to be marked by the solution and suitable for being incorporated in the object to be marked.

18. Kit according to claim 17, wherein the medium is made of a substance selected from the group consisting of nitrocellulose;

nylon nylon which is positively charged;

paper;

card;

wood;

plastic material;

cloth;

an organic substance in the form of a bead or a gel; and

an inorganic substance.

19. Kit according to claim 17, wherein the medium is incorporated in a sheet of a document by a means selected from the group consisting of a stamp and a hologram strip.

20. Kit according to claim 14, wherein the chemical agent is specific to the target nucleic acid.

21. Kit according to claim 14, wherein the chemical agent is non-specific for the target nucleic acid.

25. Kit according to claim 16, wherein said automated machine is an ink jet machine.

26. Kit according to claim 18, wherein said inorganic substance is metal, glass, ceramics, or earthenware.