Protein that inhibits production of human choriogonadotropin

• US 5,140,100 A
• Filed: 12/28/1990
• Issued: 08/18/1992
• Est. Priority Date: 12/28/1990
• Status: Expired due to Fees

• Current Assignee: Cedars-Sinai Medical Center (Cedars Sinai Health System)
• Original Assignee: Cedars-Sinai Medical Center (Cedars Sinai Health System)
• Inventors: Braunstein, Glenn A., Ren, Song-Guang
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): Walsh, Stephen
• Application Number:
  US07/639,249
• Time in Patent Office: 599 Days
• Field of Search: 514/2, 514/21, 530/300, 530/350, 530/850, 530/851, 530/853, 530/398, 530/399, 436/65, 436/86, 436/814, 436/818
• US Class Current: 530/300
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/4703 Inhibitors; Suppressors
• CPC Class Codes: C07K 14/575 Hormones derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin C07K14/665, e.g. corticotropin C07K14/695
• CPC Class Codes: Y10S 436/814 Pregnancy
• CPC Class Codes: Y10S 436/818 Human chorionic gonadotropin
• CPC Class Codes: Y10S 530/85 Reproductive organs or embryos
• CPC Class Codes: Y10S 530/851 Placenta; amniotic fluid
• CPC Class Codes: Y10S 530/853 Ovary; eggs; embryos

Abstract

A substance having an inhibitory effect on the production of human choriogonadotropin (hCG) is described, which comprises a protein isolated from human decidual cells with a molecular weight of about 7,000 to about 10,000 daltons, and useful for use in inhibiting production of hCG in vivo or in vitro or may be measured as an indication of a cause of hCG inhibition.

Claims

1. A substance having an inhibitory effect on the production of human chorionic gonadotropin (hCG) comprising a regulatory polypeptide being characterized in that it:

(a) inhibits, in a dose dependent manner, the production of hCG by human trophoblasts in vitro;

(b) substantially inhibits the stimulation of hCG secretion by human trophoblasts caused by exogenous cyclic AMP in vitro;

(c) does not substantially inhibit the production of human placental lactogen (hPL) by human trophoblasts in vitro; and

(d) exhibits a molecular weight of about 7,000 to about 10,000 daltons as determined by ultrafiltration and gel exclusion chromatography.

2. A substance having an inhibitory effect on the production of human chorionic gonadotropin (hCG) comprising a regulatory polypeptide isolated from human decidual cells said regulatory polypeptide being characterized in that it:

(a) inhibits, in a dose dependent manner, the production of hCG by human trophoblasts in vitro;

(b) substantially inhibits the stimulation of hCG secretion by human trophoblasts caused by exogenous cyclic AMP in vitro;

(c) does not substantially inhibit the production of human placental lactogen (hPL) by human trophoblasts in vitro; and

(d) exhibits a molecular weight of about 7,000 to about 10,000 daltons as determined by ultrafiltration and gel exclusion chromatography.