Multi-sample filtration plate assembly

• US 5,141,719 A
• Filed: 07/18/1990
• Issued: 08/25/1992
• Est. Priority Date: 07/18/1990
• Status: Expired due to Fees

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Geisselsoder, Janet, Fernwood, George G., Penaluna, William A.
• Primary Examiner(s): Johnston, Jill A.
• Assistant Examiner(s): Ludlow, Jan M.
• Application Number:
  US07/555,139
• Time in Patent Office: 769 Days
• Field of Search: 422/101, 435/311, 435/299-301, 210/955, 210/323.1
• US Class Current: 422/534
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01L 3/50255 Multi-well filtration

Abstract

A plate assembly for performing multiple filtrations with the use of a vacuum offers advantages for small samples due to the construction of its component plates, which include an upper plate with apertures forming sample wells, a membrane sheet forming the floor of each well, a collection plate with individual wells underneath the membrane sheet, aligned with the upper plate apertures, and a drop guide plate between the membrane sheet and the collection plate to guide and force small quantities of liquid downward from the membrane into the collection wells. The arrangement permits extremely small quantities of species on the membrane to be drawn into the collection wells without dilution, and avoids cross contamination between collection wells.

Claims

1. A plate assembly for performing filtration on a plurality of samples, said assembly comprising:

an upper plate having a plurality of apertures;

a single sheet of porous material of sufficient dimensions to span the entire plurality of said apertures;

a rigid single-piece drop guide plate with a plurality of tubes incorporated therein, said tubes extending through said drop guide plate and aligned with said apertures, each said tube having an inner diameter of less than about 0.1 inch and terminating in a raised land of diameter exceeding the diameter of each of said apertures;

a drop guide support plate having a recess to receive said drop guide plate, said recess surrounded by a peripheral shoulder, said drop guide support plate having a plurality of passages therethrough, one aligned with each of said apertures, to permit said tubes of said drop guide plate to extend therethrough;

clamping means for clamping said upper plate to said peripheral shoulder of said drop guide support plate such that said drop guide support plate will force said drop guide plate against the underside of said upper plate and thereby force said raised lands against regions on the underside of said upper plate surrounding each of said apertures;

a lower plate having a plurality of wells aligned with said apertures and said tubes;

said upper plate, drop guide plate and lower plate constructed to fit together in a manner permitting the placement of one end of each of said tubes against the underside of said porous membrane at the closed end of one of said apertures with the other end of each of said tubes extending into the interior of one of said wells; and

means for drawing a vacuum through said upper plate, drop guide plate and lower plate when so joined, to draw liquid from said apertures through said tubes into said wells.

2. A plate assembly in accordance with claim 1 in which each said tube has an inner diameter of from about 0.05 inch to about 0.08 inch.

3. A plate assembly in accordance with claim 1 further comprising a base block with a chamber therein, and means for supporting said lower plate in said chamber.

4. A plate assembly in accordance with claim 1 in which said clamping means comprises:

only one gasket sheet interposed between said upper plate and said drop guide support plate, said gasket sheet having holes therein aligned with said apertures, the diameter of said holes approximately equal to that of said apertures;

and said upper plate, drop guide plate and drop guide support plate are constructed to fit together in a manner permitting said upper plate and said drop guide support plate to be clamped together with said sheet of porous material, said gasket sheet and said drop guide plate in between, thereby compressing said upper plate, said sheet of porous material, said gasket sheet and said drop guide plate together.

5. A plate assembly in accordance with claim 4 in which said upper plate, said drop guide plate and said drop guide support plate are collectively defined as a subassembly, and said plate assembly further comprises a base block with a recess in the upper surface thereof, said base block sized to support said subassembly above said recess, and means in said recess for supporting said lower plate therein.

6. A plate assembly in accordance with claim 4 in which said upper plate, said drop guide plate and said drop guide support plate are each substantially rectangular, and said upper plate and said drop guide support plate both exceed said drop guide plate in at least one dimension selected from length and width.

7. A plate assembly in accordance with claim 6 in which said plate assembly further comprises bolting means for bolting said upper plate to said drop guide support plate with said sheet of porous material and said drop guide plate in between, with bolts positioned to bypass said sheet of porous material and said drop guide plate.

8. A plate assembly for performing filtration on a plurality of samples, said assembly comprising:

an upper plate having a plurality of apertures arranged therein in an array;

a porous membrane sheet spanning said array of apertures;

only one gasket sheet, said gasket sheet having a plurality of holes therein aligned with said apertures;

a rigid single-piece drop guide plate with a plurality of tubes incorporated therein, extending therethrough and aligned with said apertures, each said tube having an inner diameter of from about 0.05 inch to about 0.08 inch, a length of from about 0.25 inch to about 1.0 inch, and terminating at an upper end in a raised land;

a drop guide support plate sized to receive said drop guide plate and having at least one opening therein to permit passage of said tubes;

means for clamping said drop guide support plate to said upper plate with said raised lands compressing said porous membrane sheet and said gasket sheet against the underside of said upper plate;

a lower plate having a plurality of wells aligned with said apertures and said tubes;

a base block having an upper surface with a recess therein, said base block constructed to support said drop guide support plate above said recess and containing means in said recess for supporting said lower plate therein and means for drawing a vacuum in said recess.