Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis

• US 5,141,813 A
• Filed: 08/28/1989
• Issued: 08/25/1992
• Est. Priority Date: 08/28/1989
• Status: Expired due to Term

• Current Assignee: Clontech Laboratories Incorporated
• Original Assignee: Clontech Laboratories Incorporated
• Inventors: Nelson, Paul S.
• Primary Examiner(s): Brown, Johnnie R.
• Assistant Examiner(s): Kunz, Gary L.
• Application Number:
  US07/399,658
• Time in Patent Office: 1,093 Days
• Field of Search: 536/27, 536/28, 536/29, 536/23, 435/6, 501/33, 560/155, 428/402
• US Class Current: 428/402
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: Y10T 428/2982 Particulate matter [e.g., sphere, flake, etc.]

Abstract

The subject invention concerns a novel multifunctional solid support reagent which is useful in solid phase oligonucleotide synthesis. Specifically, the reagent is used in a solid phase oligonucleotide process to chemically modify the 3'"'"' terminus of a synthetic oligonucleotide with any chemical functional group. The invention reagent can be used to construct 3'"'"' labeled oligonucleotide hybridization probes to detect the presence of a target polynucleotide in biological and clinical samples.

Claims

1. A compound having the following structure:

##STR5## wherein W is alkylamine controlled pore glass, wherein said alkylamine has 1 to 50 carbon atoms.

2. A non-isotopic oligonucleotide labeling kit comprising the following separately compartmentalized components:

(a) a composition comprising a compound having the following structure;

##STR6## wherein W is alkylamine controlled pore glass, wherein said alkylamine has 1 to 50 carbons; and

(b) an appropriate reporter molecule selected from the group consisting of biotin and fluorescein.