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Automated bioluminescence microbial monitor

  • US 5,141,869 A
  • Filed: 06/27/1990
  • Issued: 08/25/1992
  • Est. Priority Date: 06/27/1990
  • Status: Expired due to Fees
First Claim
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1. A microbial monitor, which comprises:

  • a. a bladder for enriching microbial concentration in a liquid sample constructed so as to expand to a volume commensurate with a volume of said liquid sample and a volume of growth buffer introduced to said bladder, wherein said construction enables zero gravity compatibility;

    b. a means in flow communication with said bladder for introducing the liquid sample to said bladder;

    c. a second means in flow communication with said bladder for introducing the growth buffer to said bladder;

    d. a bacteria filter located within said blader for concentrating the amount of microbes in the liquid sample, wherein said concentrated microbes grow in said growth buffer to become enriched in concentration;

    e. a luciferin/luciferase assembly in flow communication with said bladder for containing luciferin/luciferase and for receiving bacteria release agent and a sample of said enriched microbial concentration from said bladder;

    f. a bacteria release agent reservoir in flow communication with said luciferin/luciferase assembly, for supplying the bacteria release agent to said luciferin/luciferase assembly;

    g. a means for mixing the luciferin/luciferase with the bacteria release agent and the enriched microbial concentration thereby causing the emission of light; and

    h. a means for measuring said emitted light, wherein said means for measuring said emitted light is connected to said luciferin/luciferase assembly.

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