Fluid dispensing system with optical locator

• US 5,141,871 A
• Filed: 05/10/1990
• Issued: 08/25/1992
• Est. Priority Date: 05/10/1990
• Status: Expired due to Fees

• Current Assignee: Behringwerke Aktiengesellschaft (Sanofi )
• Original Assignee: PB Diagnostic Systems, Inc. (Sanofi )
• Inventors: Singh, Shailendra, Kureshy, Fareed, Long, Ernest W.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): REDDING, DAVID A
• Application Number:
  US07/521,364
• Time in Patent Office: 838 Days
• Field of Search: 73/864.21, 73/864.24, 73/864.25, 356/375, 422/63-67, 422/100, 422/, 422/68.1, 422/82.05, 422/82.08, 422/82.09, 436/44-49, 436/179-180
• US Class Current: 436/47
• CPC Class Codes: G01N 2035/0475 electric, e.g. stepper motor, solenoid
• CPC Class Codes: G01N 2035/1013 Confirming presence of tip
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1083 with one horizontal degree of freedom
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

There is described an analytical isntrument for the analysis of fluids such as biological fluids. The instrument includes a fluid dispensing system having a pipette for dispensing fluid test samples and test reagents to assay cartridges. An optical detection system provides a light beam which intercepts a downward travel path of the pipette prior to its reaching the dispense position. Measurement of the intensity of the light beam in conjunction with the position of the pipette enables a controller to indicate the presence or absence of a pipette tip. In addition, interception of the light beam by the pipette initiates a final approach sequence for the pipette to the dispense position above an assay cartridge by a predetermined increment of travel.

Claims

1. A method for dispensing fluid to an assay element in an analytical instrument including a pipette, the instrument comprising transport means for advancing the pipette between s reservoir holding a fluid, the assay element and a supply of disposable pipette tips adapted to be affixed to a stem of the pipette, the method comprising:

(a) affixing a tip on the stem and aspirating fluid into said tip;

(b) advancing the pipette along a predetermined path toward an assay element carried on a conveyor;

(c) designating a reference location on said path by means of a light beam which is located a predetermined distance from said conveyor;

(d) observing a breaking of the light beam by the tip during a movement of the pipette along the path, the breaking of the light beam signaling a position of said tip at said reference location;

(e) computing an additional increment of travel based on the height of said assay element above a reference surface of said conveyor; and

(f) advancing the pipette said additional increment of travel from said reference location toward the assay element prior to dispensing the fluid to the assay element.

2. A method according to claim 1 further comprising a step of:

setting a second reference location beyond said first mentioned reference section along said path of travel; and

wherein, upon a failure to observe the breaking of the light beam in said observing step (d), said advancing step (b) is continued until said pipette reaches said second reference location.

4. A method according to claim 1 wherein said step of observing includes measuring the intensity of said light beam, a fully obscured light beam indicating that said pipette tip is present.

5. A method according to claim 2 further comprising the steps of retracting said pipette and signaling a fault in the test system.

3. A method according to claim further comprising the steps of:

dispensing the fluid to said assay element; and

retracting said pipette from said assay element.

6. An analytical instrument in combination with a reservoir storing a fluid and employing a pipette for dispensing the fluid to an assay element, the instrument comprising:

a pipette and a supply of disposable pipette tips to be affixed to a stem of the pipette;

a conveyor for carrying an assay element;

transport means for advancing the pipette to said supply of disposable pipette tips for replenishment of a tip, the transport means advancing the pipette between the reservoir and an assay element for dispensing the fluid; and

wherein the analytical instrument further comprises an optical detection system which provides a light beam at a predetermined position relative to said conveyor for sensing the location of said pipette tip, the detection system providing a location signal indicating the presence of said tip at a reference location on a path of travel of the tip toward an assay element, the reference location being distant from the assay element; and

said transport means moving said pipette to the reference location, said instrument including means responsive to the location signal for computing an additional distance of travel based on the height of an assay element above a reference surface of said conveyor and for advancing said pipette tip from said reference location by said additional distance for placing said tip in a position for dispensing the fluid to an assay element.

7. An analytical instrument according to claim 6 wherein

said transport means in moving said pipette beyond said reference location to a second reference location on said travel path in the absence of an interruption of said light beam;

and

said means responsive to said location signal is further responsive to the location of said pipette on said travel path for signaling the absence of said tip on said stem upon the attainment of said second reference location by said pipette without interruption of said light beam.