Apparatus for counting particles suspended in a fluid having a polarizing beam splitter
First Claim
1. An apparatus for counting particles suspended in a fluid, comprising:
- a flow cell;
fluid supplying means for supplying said flow cell with a fluid containing sample particles;
optical means for applying an irradiation beam to said sample particles in said fluid;
light gathering means for forming an image of the sample particles;
light sensing means for sensing light from the formed image of the sample particles; and
analyzing means for analyzing said sample particles on the basis of the sensed light;
wherein the optical means includes at least one polarizing beam splitter.
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Accused Products
Abstract
An apparatus for counting and analyzing sample particles such as cells suspended in a fluid by applying an irradiation beam such as a laser beam to the sample particles in a flow cell. The apparatus includes a device for supplying a flow cell with suspension of sample particles, an optical system for applying a laser beam to the sample particles in the above-mentioned suspension, a light gathering device for forming image of the above-mentioned sample particles, a light sensing device for sensing light from the above-mentioned formed image of the sample particles, and an analysis for analyzing the above-metnioned sample particles on the basis of the above-mentioned sensed light. The optical system may include a polarizing beam splitter and such convergent lenses having mutually different magnifications relative to two directions perpendicular to the optical axis of the laser beam.
50 Citations
20 Claims
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1. An apparatus for counting particles suspended in a fluid, comprising:
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a flow cell; fluid supplying means for supplying said flow cell with a fluid containing sample particles; optical means for applying an irradiation beam to said sample particles in said fluid; light gathering means for forming an image of the sample particles; light sensing means for sensing light from the formed image of the sample particles; and analyzing means for analyzing said sample particles on the basis of the sensed light; wherein the optical means includes at least one polarizing beam splitter. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19)
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2. An apparatus for counting particles suspended in a fluid, comprising:
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a flow cell; fluid supplying means for supplying said flow cell with a fluid containing sample particles; optical means for applying an irradiation beam to said sample particles in said fluid; light gathering means for forming an image of the sample particles; light sensing means for sensing light from the formed image of the sample particles; and analyzing means for analyzing said sample particles on the basis of the sensed light; wherein said optical means includes at least one polarizing beam splitter, and two convergent lenses having mutually different magnifications with respect to two directions perpendicular to an optical axis of said irradiation beam, said two convergent lenses being disposed between said at least one polarizing beam splitter and said flow cell. - View Dependent Claims (20)
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14. An apparatus for counting particles suspended in a fluid according to clam 7, wherein said light gathering means includes a light gathering lens, and said light sensing means includes a light sensor.
Specification