Optical fiber sensors for continuous monitoring of biochemicals and related method
First Claim
1. A sensor for sensing properties of sample analyte, for use with light source means and detection means operatively associated with an optical fiber, comprising:
- a porous membrane, permeable to said analyte,porous reflective surface means adjacent to said porous membrane, for defining a chamber therebetween,dye-labeled analog-analyte in solution contained in said chamber,receptor material disposed within said chamber, and said receptor material being capable of chemically binding with said analyte and said analog-analyte, andtransparent retention means wrapped across a wall of said reflective surface means facing out of said chamber such that said transparent retention means traps free dye-labeled analog-analyte which passes through said reflective surface means, and said retention means is transparent to an emission signal generated by fluorescence of said dye-labeled analog-analyte when said dye-labeled analog-analyte is excited with light.
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Abstract
A device and method for measuring properties of certain substances such as analytes are provided. The device includes a probe housing having an optical fiber associated therewith. The housing is capped on one end by a porous membrane which is permeable to the analyte being studied. The housing also includes a reflective surface member disposed between the end of the optical fiber and the membrane, thereby defining a chamber. The reflective surface member does not allow light to be transmitted into or out of the chamber. However, a dye-labeled analog-analyte can permeate the reflective surface member and thereby allow a measurement to be taken which is related to the concentration of the analyte being studied. An embodiment for use in vivo is provided. A related method is also provided.
92 Citations
17 Claims
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1. A sensor for sensing properties of sample analyte, for use with light source means and detection means operatively associated with an optical fiber, comprising:
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a porous membrane, permeable to said analyte, porous reflective surface means adjacent to said porous membrane, for defining a chamber therebetween, dye-labeled analog-analyte in solution contained in said chamber, receptor material disposed within said chamber, and said receptor material being capable of chemically binding with said analyte and said analog-analyte, and transparent retention means wrapped across a wall of said reflective surface means facing out of said chamber such that said transparent retention means traps free dye-labeled analog-analyte which passes through said reflective surface means, and said retention means is transparent to an emission signal generated by fluorescence of said dye-labeled analog-analyte when said dye-labeled analog-analyte is excited with light.
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2. A sensor for optically measuring properties of at least one sample analyte, comprising
a probe housing, optical fiber means for transmitting light energy into and out of said housing, one end of said optical fiber means disposed within a hollow portion of said housing and spaced from one end of said probe housing, porous membrane means disposed generally adjacent said one end of said housing, for defining a chamber between an inner wall of said porous membrane means and said optical fiber means, reflective surface means disposed within said chamber between said optical fiber means and said porous membrane means, and spaced from said porous membrane means, said reflective surface means for defining an illuminated region between said one end of said optical fiber means and said reflective surface means and a dark region between said reflective surface means and said porous membrane means, a dye-labeled analog-analyte in solution disposed within said chamber, receptor material disposed within either said illuminated region or said dark region, and said receptor material being capable of chemically binding with said analyte and said analog-analyte, light source means for generating excitation energy, said light source means operatively associated with said optical fiber means such that said excitation light passes through said optical fiber means to said reflective surface means, and detection means operatively associated with said optical fiber means, for detecting an emission signal generated by said dye-labeled analog-analyte.
Specification