Polysaccharide resolving gels and gel systems for stacking electrophoresis
First Claim
Patent Images
1. An aqueous electrophoretic resolving gel composition comprising:
- (A) two hydrogels, at least one of which has been derivatized and, independently, at least one of which has been depolymerized sufficiently to reduce the casting-effective viscosity of the total resolving gel composition;
other than a combination of (i) hydroxyethyl agarose which was subsequently depolymerized and (ii) 1,2-dihydroxypropyl agarose;
(B) water; and
(C) a resolving gel buffer.
5 Assignments
0 Petitions
Accused Products
Abstract
Electrophoretic resolving gel compositions, discontinuous stacking gel systems containing these and other resolving gel compositions, and kits for preparing such gel systems, comprising one or more polysaccharide hydrogels of which agarose is typical wherein at least one of the gels in the resolving gel composition has been derivatized and at least one of the gels in the resolving gel composition has been depolymerized sufficiently to reduce its casting-effective viscosity.
-
Citations
33 Claims
-
1. An aqueous electrophoretic resolving gel composition comprising:
-
(A) two hydrogels, at least one of which has been derivatized and, independently, at least one of which has been depolymerized sufficiently to reduce the casting-effective viscosity of the total resolving gel composition;
other than a combination of (i) hydroxyethyl agarose which was subsequently depolymerized and (ii) 1,2-dihydroxypropyl agarose;(B) water; and (C) a resolving gel buffer. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
-
-
2. An electrophoretic discontinuous stacking gel system comprising:
-
(I) a resolving gel composition comprising; (A) one or more thermo- and/or pH- reversible hydrogels, at least one of which has been derivatized and, independently, at least one of which has been depolymerized sufficiently to reduce the casting-effective viscosity of said resolving gel composition; (B) water; (C) a resolving gel buffer; and (D) optionally, about 1-5 % w/w of said resolving gel, of at least one low molecular weight polyol;
in electrophoretic-gel-operative combination with;(II) a superposed or adjacent stacking gel which is discontinuous from said resolving gel as to at least one of;
pore size, ionic strength, ionic composition, or pH, comprising;(A) one or more derivatized or native thermo- and/or pH- reversible hydrogels; (B) water; and (C) a stacking gel buffer. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
-
Specification