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Double capture assay method employing a capillary flow device

  • US 5,145,784 A
  • Filed: 01/02/1992
  • Issued: 09/08/1992
  • Est. Priority Date: 05/04/1988
  • Status: Expired due to Fees
First Claim
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1. A double capture immunoassay for an antigen of interest in a sample, comprising the steps of:

  • a. combining;

         1) the sample;

         2) magnetic particles having affixed thereto antigen of interest;

         3) nonmagnetic detectable particles having affixed thereto the antigen of interest; and

         4) unbound antibody specific for the antigen of interest, to produce a reaction mixture;

    b. incubating the reaction mixture under conditions and for a period of time sufficient for antigen of interest to bind to antibody specific for the antigen;

    c. separating the magnetic particles from the nonmagnetic particles;

    d. capturing the nonmagnetic particles on a solid material; and

    e. determining the presence or absence of captured nonmagnetic particles on the solid material.f. determining the presence or absence of the antigen of interest in the sample by relating the presence or absence of captured nonmagnetic particles to a relationship between captured nonmagnetic detectable particles having affixed thereto the antigen of interest and the antigen of interest.

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