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Method for separation of anionic oligosaccharides

  • US 5,145,956 A
  • Filed: 12/11/1991
  • Issued: 09/08/1992
  • Est. Priority Date: 12/11/1991
  • Status: Expired due to Fees
First Claim
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1. A method to separate components of a mixture of anionic oligosaccharides lacking carbonyl groups, which method comprises:

  • applying said mixture to a chromatographic column wherein said chromatographic column contains a solid support coated with polyethyleneimine (PEI) under conditions where said anionic oligosaccharides are adsorbed to the column;

    followed by eluting said column with a salt gradient to obtain a multiplicity of fractions so as to separate said anionic oligosaccharide components.

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