Non-specific immune system enhancement

• US 5,147,289 A
• Filed: 03/29/1990
• Issued: 09/15/1992
• Est. Priority Date: 03/29/1990
• Status: Expired due to Term

• Current Assignee: Thereakos Incorporated
• Original Assignee: Therakos, Inc (Mallinckrodt plc)
• Inventors: Edelson, Richard L.
• Primary Examiner(s): Hafer, Robert A.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/502,083
• Time in Patent Office: 901 Days
• Field of Search: 424/90, 604/4-6, 604/20
• US Class Current: 604/6.03
• CPC Class Codes: A61K 2039/5158 Antigen-pulsed cells, e.g. T-cells
• CPC Class Codes: A61K 35/17 Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes when activated by a specific antigen A61K39/00
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/39 characterised by the immunostimulating additives, e.g. chemical adjuvants
• CPC Class Codes: A61K 41/0023 Agression treatment or altering
• CPC Class Codes: A61K 41/17 by ultraviolet [UV] or infrared [IR] light, X-rays or gamma rays
• CPC Class Codes: A61M 1/362262 Details of incorporated reservoirs
• CPC Class Codes: A61M 1/3687 Chemical treatment A61M1/3675 takes precedence
• CPC Class Codes: A61M 2202/0064 changed by biological action
• CPC Class Codes: A61M 2202/0439 White blood cells; Leucocytes lymphocytes A61M2202/0407
• CPC Class Codes: C12N 5/0636 T lymphocytes
• CPC Class Codes: Y10S 424/81 involving autoimmunity, allergy, immediate hypersensitivity, delayed hypersensitivity, immunosuppression, immunotolerance, or anergy

Abstract

The present invention pertains to a method for non-specifically enhancing the immune system response of a mammal to an antigen which comprises the steps of (a) withdrawing leukocyte containing material from the mammal, (b) treating the withdrawn leukocytes in a manner to alter the cella, and (c) returning the treated leukocytes to the mammal. The one embodiment, the method for non-specifically enhancing the immune system response of a mammal is carried out in conjunction with artificially contacting the mammal'"'"'s immune system with an antigen for a suitable period of time to stimulate the immune system. In another embodiment, the mannal'"'"'s immune system is naturally exposed to the antigen prior to the non-specific enhancement of the immune system. In yet another embodiment, the invention is directed at a method for preparing an autologous non-specific leukocyte adjuvant.

Claims

1. A method for non-specifically enhancing the immune system response of a mammal to an antigen which comprises the steps of:

(A) enhancing the immune system response by;

(a) withdrawing leukocyte containing material from the mammal,

(b) treating the withdrawn leukocytes in a manner to alter the cells,

(c) returning the treated leukocytes to the mammal, and

(B) artificially contacting the mammal'"'"'s immune system with the antigen for a suitable period of time to stimulate the immune system.

2. The method according to claim 1, wherein steps (A) and (B) are carried out simultaneously.

3. The method according to claim 1, wherein the mammal is a human.

4. The method according to claim 1, wherein the leukocyte containing material in step (A)(a) is selected from the group consisting of blood, lymph fluid, bone marrow, and lymphatic organ tissue.

5. The method according to claim 1, wherein the leukocytes in step (A)(a) are lymphocytes.

6. The method according to claim 5, wherein the lymphocytes are T/cells.

7. The method according to claim 1, wherein the treatment of the leukocytes in step (A)(b) alters the structure of the cell membranes of the leukocytes.

8. The method according to claim 1, wherein the treatment of the leukocytes in step (A)(b) alters the DNA or the cytoplasmic proteins of the leukocytes.

9. The method according to claim 1, wherein the treatment of the leukocytes in step (A)(b) comprises irradiating the leukocytes with ultraviolet radiation in the presence of a photoactivatable agent.

10. The method according to claim 9, wherein the photoactivatable agent is administered to the mammal prior to step (A)(a).

11. The method according to claim 1, wherein the antigen in step (B) is in the form of a vaccine.

12. The method according to claim 1, wherein the antigen in step (B) is associated with a deficiency in the immune system.

13. The method according to claim 12, wherein the antigen is associated with an immunodeficiency disease.

14. The method according to claim 13, wherein the immunodeficiency disease is selected from the group consisting of acquired immunodeficiency syndrome (AIDS), cancer, immunodeficiency of old age, and immunodeficiency following immunosuppressive therapy.

15. The method according to claim 1, wherein the antigen in step (B) is associated with a hypersensitivity disease.

16. The method according to claim 1, wherein the hypersensitivity disease is selected from the group consisting of delayed-type hypersensitivity reaction, autoimmune disease, allergy, infectious disease, rejection of allografts and graft-versus-host reaction.

17. The method according to claim 9, wherein step (A) (b) comprises the steps of:

(1) forming the withdrawn leukocytes into an extracorporeal stream,

(2) flowing the stream through a treatment chamber substantially transparent to ultraviolet radiation, and

(3) irradiating the stream in the chamber with ultraviolet radiation in the presence of the photoactivatable agent.

18. The method according to claim 17, wherein the withdrawing of leukocyte containing material, the treating of the withdrawn leukocytes, and the returning of the treated leukocytes to the mammal is carried out as a continuous operation.

19. A method for improving vaccine therapy in a mammal, comprising:

a) withdrawing leukocyte containing material from the mammal prior to administration of a vaccine to the mammal;

b) treating the withdrawn leukocytes in a manner to alter the cells;

c) returning the treated leukocytes to the mammal; and

d) administering the vaccine to the mammal.