Preservation of viruses

• US 5,149,653 A
• Filed: 11/20/1989
• Issued: 09/22/1992
• Est. Priority Date: 01/21/1988
• Status: Expired due to Term

• Current Assignee: Quadrant Drug Delivery Limited (Vectura Group Ltd.)
• Original Assignee: Quadrant Bioresources Ltd.
• Inventors: Roser, Bruce J.
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): REARDON, TIMOTHY
• Application Number:
  US07/411,473
• Time in Patent Office: 1,037 Days
• Field of Search: 435/260, 435/235, 435/948
• US Class Current: 435/260
• CPC Class Codes: A61K 2039/5252 inactivated (killed)
• CPC Class Codes: A61K 2039/70 Multivalent vaccine
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61K 39/13 Poliovirus
• CPC Class Codes: A61K 39/23 Parvoviridae, e.g. feline panleukopenia virus
• CPC Class Codes: A61K 39/245 Herpetoviridae, e.g. herpes simplex virus
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: C12N 2750/14334 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2760/16051 Methods of production or purification of viral material
• CPC Class Codes: C12N 2760/16134 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2770/32634 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 2770/32651 Methods of production or purification of viral material
• CPC Class Codes: C12N 2795/10351 Methods of production or purification of viral material
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
• CPC Class Codes: Y10S 435/948 using viruses or cell lines

Abstract

A method of preserving live viruses comprises subjecting an aqueous system containing the virus to drying either in the frozen state or at ambient temperature, in the presence of trehalose.

Claims

1. A method of preserving an infectious virion for subsequent reconstitution, the method comprising subjecting an aqueous system containing the infectious virion and trehalose to drying either in the frozen state or at ambient temperature so as to produce a preserved infectious virion, whereby upon rehydration said preserved infectious virion is a reconstituted infectious virion.

2. A method according to claim 1 in which the aqueous system contains 1 to 20% by weight of trehalose.

3. A method according to claim 1 in which the aqueous system contains 5 to 20% by weight of trehalose.