Methods and apparatuses for preparative electrophoresis

• US 5,151,165 A
• Filed: 04/25/1990
• Issued: 09/29/1992
• Est. Priority Date: 12/11/1984
• Status: Expired due to Fees

• Current Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH)
• Original Assignee: Institut National De La Sante Et De La Recherche Medicale (Inserm) (Allianz Global Investors GmbH)
• Inventors: Huynh, Van T.
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Koestner, Caroline
• Application Number:
  US07/513,029
• Time in Patent Office: 888 Days
• Field of Search: 204/299 R, 204/182.8, 204/182.4
• US Class Current: 204/615
• CPC Class Codes: B01D 57/02 by electrophoresis treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones

Abstract

The electrophoresis apparatus of the invention includes a semi-permeable element 6 which makes it possible to take advantage of the phenomenon of electro-endosmosis which is generated during electrophoresis, and outlet tube(s) 15 to transfer the products which emerge from the electrophoresis gel to the exterior by means of a buffer 9 which flows across the semi-permeable element in the direction opposite to that in which the products migrate.

Claims

1. An apparatus for preparative electrophoresis which does not include a pump consisting essentially of

a first zone containing an upper electrode and a first electrode buffer system;

a second zone containing a lower electrode and a second electrode buffer system;

an enclosed means having upper and lower ends connecting said first and second zones, the upper and lower ends of said enclosed means being immersed in said first and second electrode buffer systems, respectively;

a continuous separation gel having an upper and lower surface in said enclosed means the upper surface of the gel on which is placed the sample containing the product to be recovered;

a semi-permeable element having an electroendosmotic capacity maintained tightly and parallel to the lower gel surface;

an elution chamber defined by the lower surface of said gel and said semi-permeable element;

at least one outlet for the removal of products which emerge from the gel, without using a pump, by means of the buffer from the second electrode buffer system flowing in the direction opposite to that of the migration of the products to be separated across said semi-permeable element, said outlet being located between the gel and said semi-permeable element, and being with the gel and the molecules to be separated on the same side of the semi-permeable element, the gel and said semi-permeable element being separated by less than one millimeter.

2. Apparatus according to claim 1, characterized in that at least one outlet is used and is arranged alongside the gel or at the center of the gel surface in such a way that all of the molecules of a given type of product which emerge from the gel are removed at the same time and as quickly as possible.

3. Use of an apparatus according to claim 1, for the purification of a chemical or biological composition.

4. Use of an apparatus according to claim 1 for the purification of proteins.

5. The use according to claim 4 wherein the proteins are selected from the group comprising enzymes and nucleic acids.