Immunoassay apparatus

• US 5,152,962 A
• Filed: 07/22/1988
• Issued: 10/06/1992
• Est. Priority Date: 07/22/1988
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: ORD Corporation
• Inventors: Lackie, Steve J.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Alexander, Lyle
• Application Number:
  US07/223,154
• Time in Patent Office: 1,537 Days
• Field of Search: 422/57, 422/68, 422/58, 436/800, 436/805, 436/807, 436/172, 356/317, 356/318, 250/459.1, 250/461.1, 250/461.2, 350/96.15, 350/96.18
• US Class Current: 422/68.1
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures

Abstract

An assay apparatus employing total internal reflection of excitation radiation at the interface between a replaceable optically conductive assay device and a surrounding liquid phase containing the substance being assayed. The assay device is formed of a radiation transmissive, elongated fiber having on the surface thereof a component of a complex formed in an immunological-type specific reaction. A fluorophore that can be excited into fluorescence by the excitation radiation is attached to another component of the complex. The assay device also includes a boss formed of a similarly radiation transmissive material with an index of refraction preferably matched to that of the fiber, one end of the fiber being fixedly coupled to the boss. The boss is dimensioned so that the maximum solid acceptance angle for excitation radiation introduced into the fiber end lies totally within the transmissive medium of said boss. In one embodiment, the fiber is coaxially mounted within a tube that is sized with respect to the fiber so that a fluid sample may be introduced into the tube.

Claims

1. Apparatus for assaying a fluid sample with excitation radiation from a radiation source, which radiation is capable of exciting fluorescence in fluorescent material, said apparatus comprising:

a totally internally reflecting, unitary elongated substrate transmissive to both said excitation radiation and to said fluorescence, said substrate comprising a boss and an elongated fiber into one end of which said radiation is to be introduced and at least a portion of the surface of which is adapted to contact said sample;

said one end of said fiber being fixedly coupled to said boss, said boss being dimensioned so that the maximum solid acceptance angle for said excitation radiation introduced into said one end lies totally within the transmissive medium of said boss.

2. Apparatus as defined in claim 1 wherein the juncture of said fiber and boss presents no optical discontinuity to propagation of said radiation down said fiber.

3. Apparatus as defined in claim 1 wherein said boss has an index of refraction matched to that of said fiber.

4. Apparatus as defined in claim 1 including a coating of said fluorescent material on at least a portion of the surface of said fiber.

5. Apparatus as defined in claim 4 where said fluorescent material includes at least a moiety of an antibody-antigen complex that includes a tag that will provide said fluorescence when excited by an evanescent wave generated by said excitation radiation.

6. Apparatus as defined in claim 1 including a hollow elongated enclosure disposed about and spaced from said fiber so as to provide an interspace between said fiber and said enclosure.

7. Apparatus as defined in claim 6 wherein said fiber is disposed concentrically within said enclosure.

8. Apparatus as defined in claim 6 wherein the interspace between said enclosure and said fiber is of capillary dimensions.

9. Apparatus as defined in claim 6 wherein one end of said elongated enclosure abuts and is substantially sealed to said boss adjacent the juncture of said fiber and said boss.

10. Apparatus as defined in claim 9 including conduit means extending between the exterior of said apparatus and the interspace between said enclosure and said fiber adjacent said boss.

11. Apparatus as defined in claim 1 including pumping means so connected to said interspace as to provide a fluid-sealed system for flowing said sample through said interspace.

12. Apparatus as defined in claim 1 including means associated with the other end of said fiber for blocking propagation of excitation and fluorescent radiation and stray light through said other end.

13. In apparatus as defined in claim 12 wherein said means for blocking comprises a radiation absorber having an index of refraction substantially matched to the index of refraction of said fiber.

14. Apparatus as defined in claim 1 wherein the surface of said boss opposite the juncture of said boss and fiber is substantially planar and disposed substantially perpendicularly to the axis of elongation of said fiber.

15. Apparatus as defined in claim 1 wherein the surface of said boss opposite the juncture of said boss and fiber is configured to provide a lens surface, the optical axis of which is substantially colinear with the axis of elongation of said fiber.

16. Apparatus as defined in claim 1 wherein the minimum cross-sectional dimension of said boss transverse to the axis of elongation of said fiber is substantially greater than the maximum cross-sectional diameter of said fiber.

17. Apparatus as defined in claim 1 including:

seating means for holding said boss in a fixed position;

a source of said excitation radiation;

optical means for focussing said radiation toward said seating means;

detector means for detecting fluorescence radiation emitted from an end of said fiber responsively to said excitation radiation; and

means for mounting said source, optical means and detector means in fixed relation to said seating means for introducing said excitation radiation into said end of said fiber through said boss within said acceptance angle when said boss is held in said seating means.

18. Apparatus as defined in claim 17 including:

beam splitting means coupled to said means for mounting so as to direct said excitation radiation to said optical means and for directing said fluorescence to said detector means.

19. Apparatus as defined in claim 17 wherein said boss includes a substantially planar face substantially perpendicular to the longitudinal axis of said fiber and lying on a surface of said boss opposite to the juncture of said fiber and said boss.

20. Apparatus as defined in claim 17 wherein the surface of said boss opposite the juncture of said boss and fiber is configured to provide a lens surface, the optical axis of which is substantially colinear with the axis of elongation of said fiber.

21. Apparatus as defined in claim 17 wherein said boss has a cross-sectional configuration substantially matched to the shape of said seating means so that said boss can be firmly and releasably held in said seating means.

22. Apparatus as defined in claim 17 wherein said boss has a circular cross-section and a substantially uniform diameter

23. Apparatus as defined in claim 17 including pumping means so connected to said interspace as to provide a fluid-sealed system for flowing said sample through said interspace.