Binder composition and analytical element having stabilized peroxidase in layer containing the composition
First Claim
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1. An analytical element comprising an absorbent carrier material, said element divided into two or more discrete zones, and containing in one of said zones a peroxidase-labeled ligand analog for an immunologically reactive ligand uniformly distributed in a water-soluble binder composition comprising at least about 50 percent, by weight, of poly(vinyl alcohol).
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Abstract
An analytical element has a peroxidase-labeled ligand analog distributed within a water-soluble binder composition comprising at least about 50 percent, by weight, of poly(vinyl alcohol). As a result, the peroxidase retains more of its stability prior to use. Such elements can be used to determine any of a number of immunologically reactive analytes, such as digoxin.
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Citations
19 Claims
- 1. An analytical element comprising an absorbent carrier material, said element divided into two or more discrete zones, and containing in one of said zones a peroxidase-labeled ligand analog for an immunologically reactive ligand uniformly distributed in a water-soluble binder composition comprising at least about 50 percent, by weight, of poly(vinyl alcohol).
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7. A multilayer analytical element comprising a nonporous support having thereon, in order,
a registration layer, a water-soluble layer containing a peroxidase-labeled ligand analog for an immuno-logically reactive ligand uniformly distributed in a water-soluble binder composition comprising at least about 50 percent, by weight, of poly(vinyl alcohol), and a porous spreading layer, said element further comprising an interactive composition which is capable of interacting with said ligand analog to provide a spectrophotometric signal in the presence of a substrate for peroxidase.
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12. A method for the determination of an immunologically reactive ligand comprising the steps of:
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A. in the presence of a receptor for said ligand, contacting a sample of a liquid suspected of containing said ligand with an analytical element comprising an absorbent carrier material, said element divided into two or more discrete zones, and containing in one of said zones a peroxidase-labeled ligand analog for said ligand uniformly distributed in a water-soluble binder composition comprising at least about 50 percent, by weight, of poly(vinyl alcohol), said contacting carried out in such a manner as to form a complex of receptor and ligand analog, and B. determining the amount of said ligand as a result of the presence of complexed or uncomplexed ligand analog. - View Dependent Claims (13, 14, 15, 16, 17, 18)
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19. A multilayer analytical element comprising a nonporous support having thereon, in order,
a porous spreading layer, and a water-soluble layer containing a peroxidase-labeled ligand analog for an immunologically reactive ligand uniformly distributed in a water-soluble binder composition comprising at least about 50 percent, by weight, of poly(vinyl alcohol).
Specification