Horseshoe crab amebocyte lysate factor G activation inhibitor

• US 5,155,032 A
• Filed: 05/01/1990
• Issued: 10/13/1992
• Est. Priority Date: 09/01/1988
• Status: Expired due to Term

• Current Assignee: Seikagaku Kogyo Company Limited
• Original Assignee: Seikagaku Kogyo Company Limited
• Inventors: Takahashi, Shoji, Aketagawa, Jun, Tanaka, Shigenori, Tamura, Hiroshi, Shibata, Yuko, Ohki, Makoto
• Primary Examiner(s): Brown, Johnnie R.
• Assistant Examiner(s): White, Everett
• Application Number:
  US07/474,057
• Time in Patent Office: 896 Days
• Field of Search: 536/1.1, 436/502, 436/501, 435/4, 435/23, 435/184, 435/18
• US Class Current: 435/184
• CPC Class Codes: C07H 3/06 Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
• CPC Class Codes: C08B 37/0024 beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
• CPC Class Codes: G01N 33/579 involving limulus lysate

Abstract

This invention relates to a horseshoe crab amebocyte lysate factor G activation inhibitor comprising as an active ingredient a polyglycoside containing at least one poly-(1→3)-β-D-glucoside structure portion consisting of 2 to 370 (1→3)-β-D-glucoside structural units of the following formula ##STR1## which are continuously bound to one another. This inhibitor is useful for inhibiting the activation of factor G which may exist in horseshoe crab amebocyte lysate used in the Limulus test.

Claims

1. A method of inhibiting the activation of factor G contained in horseshoe crab amebocyte lysate, which comprises adding to horseshoe crab amebocyte lysate a polyglycoside comprising a poly-(1→

3)-β

-D-glucoside structural portion consisting of 2 to 370 (1→

3)-β

-D-glucoside structural units of the following formula;

##STR16## which are continuously bound to one another, in an amount effective to inhibit the activation of factor G.

2. The method of claim 1 in which the poly-(1→

3)-62 -D-glucoside structural portion consists of 3 to 310 (1→

3)-β

-glucoside structural units of formula (1) bound to one another.

3. The method of claim 1 in which the poly-(1→

3)-62 -D-glucoside structural portion consists of 4 to 180 (1→

3)-β

-glucoside structural units of formula (1) bound to one another.

4. The method of claim 1 in which the polyglycoside is laminarioligosaccharide having a molecular weight of 342 to 1,638.

5. The method of claim 1 in which the polyglycoside is laminaridextrin having a molecular weight of 1,800 to 3,258.

6. The method of claim 1 in which the polyglycoside is a (1→

3)-β

-D-glucan having an average molecular weight of 2,000 to 6,000.

7. The method of claim 1 in which the polyglycoside is laminaran having an average molecular weight of 3,000 to 23,000.

8. The method of claim 1 in which the polyglycoside is sclerotan having an average molecular weight of 3,000 to 20,000.

9. The method of claim 1 in which the polyglycoside is schizophyllan having an average molecular weight of not more than 500,000.

10. The method of claim 1 in which the polyglycoside is lentinan having an average molecular weight of not more than 1,100,000.

11. The method of claim 1 in which the polyglycoside is a water-soluble fraction of bakers'"'"' yeast glucan having an average molecular weight of not more than 12,000.

12. The method of claim 1 in which the polyglycoside is lichenan having an average molecular weight of not more than 33,000.

13. The method of claim 1 in which the polyglycoside is barley β

-glucan having an average molecular weight of not more than 200,000.

14. The method of claim 1 in which the polyglycoside is partially carboxymethylated (1→

3)-β

-D-glucan or its salt (degree of substitution 0.003 to 1.0) having an average molecular weight of 40,000 to 240,000.

15. The method of claim 1 in which the polyglycoside is a partially carboxymethylated laminaran or its salt (degree of substitution not more than 1.0) having an average molecular weight of not more than 23,000.

16. The method of claim 1 in which the polyglycoside is partially methylated (1→

3)-β

-D-glucan (degree of substitution 0.003 to 1.0) having an average molecular weight of not more than 80,000.

17. The method of claim 1 in which the polyglycoside is partially sulfated laminaran or its salt (degree of substitution not more than 1.0) having an average molecular weight of not more than 23,000.

18. The method of claim 1 wherein the polyglycoside is used in an amount of at least 50 ng per ml of horseshoe crab amebocyte lysate.