Biosensors employing electrical, optical and mechanical signals

• US 5,156,810 A
• Filed: 06/15/1989
• Issued: 10/20/1992
• Est. Priority Date: 06/15/1989
• Status: Expired due to Fees

• Current Assignee: Biocircuits Corporation
• Original Assignee: Biocircuits Corporation
• Inventors: Ribi, Hans O.
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Ludlow, Jan M.
• Application Number:
  US07/366,651
• Time in Patent Office: 1,223 Days
• Field of Search: 422/82.01, 422/82.02, 422/82.03, 422/82.06, 436/806, 436/527, 436/501, 436/531, 435/291, 435/817, 204/403
• US Class Current: 422/82.01
• CPC Class Codes: B01J 2219/00734 Lipids
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

Biosensors are provided employing a thin crystalline diyne surfactant polymeric electrically conducting layer to which may be bound members of specific binding pairs. Binding of an analyte or a reagent to the specific binding pair member layer may change the electrical, optical or structural properties of the layer for measurement of analyte. The change in the polymeric layer provides for a sensitive measurement.

Claims

1. A biosensor comprising a plurality of layers:

a first electrically non-conductive support providing mechanical rigidity to said biosensor comprising at least two spaced apart electrical leads coated on a first surface of said support;

an electrically conducting organic polymerized crystalline surfactant layer coating said first surface between said electrical leads and comprising electrical contact portions in electrical contact with said electrical leads, said organic polymerized crystalline surfactant layer prepared from addition polymerizable conjugated diyne surfactant monomers of at least 12 carbon atoms and produced by the crystallization of said surfactant monomers and the polymerization of surfactant diynes to produce a conjugated polymer with each monomer having a hydrophilic terminum distal to said support;

a sealing coating over said electrical contact portions to protect said electrical contact portions from contact with water; and

an organic molecule layer bound to said organic polymerized crystalline surfactant layer at said hydrophilic termini, said organic molecule layer comprising a member of a specific binding pair for binding to a complementary member in an aqueous medium.

2. A biosensor according to claim 1, wherein said bound organic molecule layer is a layer of receptors.

3. A biosensor according to claim 1, wherein said surfactant diyne monomers have an aliphatic chain of from 6 to 100 carbon atoms.

4. A biosensor according to claim 1, wherein said organic molecule layer is bound to said surfactant layer through a complex of complementary binding members.

5. A biosensor according to claim 1, wherein said organic molecule layer is directly bound to said surfactant layer by covalent bonds.

6. A biosensor according to claim 1, wherein said support is transparent and further comprises a reflective layer on a second surface opposite said first surface.

7. A biosensor according to claim 1, wherein said organic molecule layer is a substantially oriented layer of proteins.

8. A biosensor according to claim 1, wherein said organic molecule layer is a layer of haptens.