Device for detecting and continuously measuring the concentration of oxidizable biodegradable substrates in a medium and method

• US 5,158,662 A
• Filed: 11/19/1990
• Issued: 10/27/1992
• Est. Priority Date: 11/19/1990
• Status: Expired due to Fees

• Current Assignee: Philip S. Osborne
• Original Assignee: Philip S. Osborne
• Inventors: Osborne, Philip S.
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): Phasge, Arun S.
• Application Number:
  US07/615,759
• Time in Patent Office: 708 Days
• Field of Search: 204/153.12, 204/153.2, 204/403, 204/284, 204/294, 204/153.16, 435/4, 435/29, 435/817
• US Class Current: 204/403.15
• CPC Class Codes: G01N 33/1866 using microorganisms
• CPC Class Codes: G01N 33/1886 using probes, e.g. submersible probes, buoys
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A device and method for detecting and/or continuously measuring the concentration of oxidizable biodegradable substrates in a medium. The device comprises an oxygenated first electrode and a second electrode. The first electrode is capable of carrying microorganisms capable of oxidizing the substrates in the presence of oxygen thus releasing electrons. The electrons are received by the second electrode and detected and/or measured by the device. The rate of electron transfer correlates to the concentration of substrate in the medium providing a method of continuous measurement of the substrate in the medium.

Claims

1. A detection device for detecting oxidizable biodegradable substrates in a medium having microorganisms therein comprising:

a first electrode comprising;

a first tube having an upper end and a lower end and an opening extending therethrough intersecting the upper and the lower ends thereof;

a first conducting element having an upper face and a lower face, and being disposed in the opening in the first tube and positioned at the lower end of the first tube, the first conducting element being porous, the first conducting element being positioned in the tube so that the lower face of the first conducting element is exposed to the medium when the first electrode is at least partially immersed in the medium;

a first conductor connected to the first connecting element, the first conductor extending through the opening in the tube;

a second electrode;

air compressor means for passing air having oxygen therein into the opening in the first tube, a portion of the compressed air passing through the first conducting element and the compressed air in the first tube substantially preventing the medium from passing through the first conducting element and into the opening in the first tube, the microorganisms in the medium associated with the first conducting element oxidizing the biodegradable substrate in the presence of oxygen passed from the air compressor means associated with the first conducting element and causing electrons to be emitted from the first conducting element, the electrons passing through the medium and the second electrode; and

means connected to the first conductor and the second electrode for detecting electron transfer between the first and second electrodes thereby indicating the oxidizable biodegradable substrates in the medium.

2. The device of claim 1 in which the first conducting element comprises an activated carbon plug.

3. The device of claim 2 in which the activated carbon plug comprises a wood grain pattern, in which the direction of the pattern is at about a 90 degree angle to the lower face portion of the carbon plug.

4. The device of claim 1 further comprising blocking means for treating the first and second conducting elements to prevent a substantial amount of the medium from passing into the openings in the first and second tubes when the electrodes are at least partially immersed in the medium.

5. The device of claim 1 wherein the first electrode further comprises:

a first plug disposed in the opening in the first tube at the upper end of the first tube for sealingly closing the upper end of the first tube.

6. The device of claim 1 wherein the second electrode further comprises:

a second tube having an upper end and a lower end and an opening extending therethrough and intersecting the upper and the lower ends thereof;

a second conducting element having an upper face and a lower face and being disposed in the opening in the second tube and positioned at the lower end of the second tube, the second conducting element being porous, the second conducting element being positioned in the second tube so that the lower face of the second conducting element is exposed to the medium when the second electrode is at least partially immersed in the medium; and

a second conductor connected to the second conducting element, the second conductor extending through the opening in the second tube.

7. The device of claim 6 in which the second conducting element comprises an activated carbon plug.

8. The device of claim 6 wherein the second electrode further comprises:

a second plug disposed in the opening in the second tube at the upper end of the second tube for sealingly closing the upper end of the second tube.