Method for cryopreserving blood vessels
First Claim
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1. A method of maintaining intact a layer of endothelial cells present in a blood vessel during cryopreservation comprising:
- i) contacting said blood vessel with an effective amount of a cryoprotectant composition comprising a medium for freezing said blood vessel, a cryoprotectively effective concentration of a cell penetrating cryoprotectant and a cryoprotectively effective concentration of a glycosaminoglycan; and
ii) maintaining said blood vessel in contact with said composition at a temperature below -100°
C.
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Abstract
A device for use in cryopreservation of blood vessels comprising a pair of stylets insertable into the ends of a dissected blood vessel segment. The stylets are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant.
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Citations
11 Claims
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1. A method of maintaining intact a layer of endothelial cells present in a blood vessel during cryopreservation comprising:
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i) contacting said blood vessel with an effective amount of a cryoprotectant composition comprising a medium for freezing said blood vessel, a cryoprotectively effective concentration of a cell penetrating cryoprotectant and a cryoprotectively effective concentration of a glycosaminoglycan; and ii) maintaining said blood vessel in contact with said composition at a temperature below -100°
C. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification